Interferon-gamma suppresses melanogenesis in B16 melanoma cells and primary human melanocytes

Inflammatory cytokines are closely related to melanogenesis in melanocytes. Here, I studied whether IFN-γ affects melanogenesis, and if so, what is the underlying mechanism involved in it. Using B16 melanoma cell lines and primary human melanocytes, I demonstrated that alpha-MSH induced tyrosinase expression and activity and TRP-1, TRP-2 expression were suppressed by IFN-γ, which resulted from the inhibition of MITF expression, upstream molecule of tyrosinase. Moreover, the inhibitory effects on MITF expression by IFN-γ occured through the disturbance of CREB binding ability on MITF promoter by reducing the binding of CBP and CREB. These results suggest that IFN-γ decreases melanogenesis through inhibiting the binding of CBP and CREB and block the MITF and tyrosinase expression. Inflammatory cytokines are closely related to melanogenesis in melanocytes. Here, I studied whether IFN-γ affects melanogenesis, and if so, what is the underlying mechanism involved in it. Using B16 melanoma cell lines and primary human melanocytes, I demonstrated that alpha-MSH induced tyrosinase expression and activity and TRP-1, TRP-2 expression were suppressed by IFN-γ, which resulted from the inhibition of MITF expression, upstream molecule of tyrosinase. Moreover, the inhibitory effects on MITF expression by IFN-γ occured through the disturbance of CREB binding ability on MITF promoter by reducing the binding of CBP and CREB. These results suggest that IFN-γ decreases melanogenesis through inhibiting the binding of CBP and CREB and block the MITF and tyrosinase expression.

Author dc:creator

• 손, 진영

Contributors dc:contributor

• 주, 일로
• 대학원 의생명과학과
• 200924471

• Melanogenesis
• a-MSH
• IFN-g
• Tyrosinase
• MITF
• STAT1
• CBP
• CREB

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000013983
000000013983

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/8592