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Showing 1 to 20 of 60 for “"Tyrosinase"”.
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Tyrosinase and Melanin Pigmentation in Azotobacter Chroococcum
Made available in DSpace on 2014-12-05T21:45:30Z (GMT). No. of bitstreams: 1 5900534.pdf: 2458013 bytes, checksum: 3302b07bb626ef937514100c5a772253 (MD5) Previous issue date: 1958
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Synthesis and activity of tyrosinase in mouse skin melanocytes
Tyrosinase (E.C. 1.14.18.1) is a key enzyme in the biosynthesis of melanin. The control of melanin sythesis was explored in skin melanocytes of the following strains; wild type (C57BL/6J-C/C) (which maximally synthesize melanin at normal mammalian body temperature, Himalayan (C57BL/6J-cʰ/cʰ) (which …
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Cell type-specific regulation of the chicken tyrosinase promoter
… in which they respond to melanogenic stimuli. Tyrosinase is the rate-limiting enzyme in the melanin synthesis pathway, and the regulation of tyrosinase gene expression in mammalian melanocytes has been extensively studied. In contrast, regulation oftyrosinase gene expression in RPE cells has …
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The cloning and characterisation of the chicken tyrosinase-related protein gene family
… the cloning and characterisation of the chicken Tyrosinase-related protein (TRP) gene family. To accomplish this goal, neural crest cells from 500 black chick embryos were cultured under conditions supportive of melanocyte differentiation and proliferation. Using RNA extracted from these …
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Insulin-mediated inhibition of tyrosinase activity and protein synthesis in melanoma cell cultures
Insulin lowers basal levels of tyrosinase activity and inhibits the MSH-stimulated rise in tyrosinase in Cloudman S-91 mouse melanoma cell cultures. These cultures are very sensitive to insulin. A concentration of insulin as low as 5 x 10 M insulin produces optimum inhibition. At maximum …
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The cellular basis of the Southern African forms of rufous & tyrosinase-positive oculocutaneous albinism
… forms of oculocutaneous described. Of these, the tyrosinasepositive and rufous forms are particularly prevalent in Southern Africa. The tyrosinase-positive form manifests as two distinctly different phenotypes with some individuals developing pigmented freckles (ephelides) on their sunexposed …
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Highly sensitive measurements of substrates and inhibitors on the basis of tyrosinase sensors and recycling systems
… Rahmen dieser Doktorarbeit wurden verschiedene Tyrosinasesensoren entwickelt, die je nach Herkunft und Eigenschaften der verwendeten Tyrosinase unterschiedliche Anforderungen erfüllen. Beispielsweise wurde einer dieser Tyrosinasesensoren für die Bestimmung phenolischer Verbindungen in Fluss- und …
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Interferon-gamma suppresses melanogenesis in B16 melanoma cells and primary human melanocytes
… I demonstrated that alpha-MSH induced tyrosinase expression and activity and TRP-1, TRP-2 expression were suppressed by IFN-γ, which resulted from the inhibition of MITF expression, upstream molecule of tyrosinase. Moreover, the inhibitory effects on MITF expression by IFN-γ occured …
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Structural analysis via X-ray crystallography of an engineered carbon methyltransferase, a phytoxic phosphonate and its modifying enzyme, and a chimeric tyrosinase
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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A study of the differentiation and dedifferentiation of three human melanoma cell lines
… proteins including, inter alia, the enzymes tyrosinase, tyrosinase-related protein-1 (TRP-1 ), tyrosinase-related protein-2 (TRP-2) and the melanosomal protein encoded by the P gene. The function of tyrosinase and TRP-2 have recently been clarified, but the roles of TRP-1 and the P protein …
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Cloning, sequencing and functional analysis of the chicken tyrosine gene promoter
… underlying lineage determination in development. Tyrosinase is a key enzyme in melanin biosynthesis and the activation of the tyrosinase gene is characteristic of differentiated melanocytes. In order to study the mechanisms underlying activation of melanocyte-specific genes during differentiation …
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Wnt Inhibitory Factor (WIF)-1 Promotes Melanogenesis in Normal Human Melanocytes
… with measurement of melanin contents, tyrosinase activity and its expression. Result: WIF-1 was expressed in melanocytes. WIF-1 inhibited proliferation of human melanocytes in a dose-dependent manner. WIF-1 also appeared to stimulate the melanin synthesis of human melanocytes. This …
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Melanocyte survival and response to treatment in Vitiligo Patients
… for their vitiligo patients. The analysis of tyrosinase and TRP-2 mRNA expression in vitiliginous skin using quantitative RT-qPCR revealed the presence of melanoblasts/melanocytes in 60% of patients (n=21). When this survival was calculated as a percentage of the mRNA expression in the …
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Birds of a white feather : a congenital hypopigmentary disorder in the chick
… Secondly, in situ hybridisation with tyrosinase and tyrosinase related protein-2 (TYRP2) probes was carried out to quantitate the number of melanocytes at different stages of development. The results indicate that tyrosinase - and TYRP2 -expressing melanocytes are present in 10-day …
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Hypomelanosis in chickens
… and a sensitive radiometric assay for tyrosinase. The results show that active tyrosinase was present in 8, 9 and 10 day skins. However, unlike normal black skin, the level of tyrosinase did not increase with age, suggesting that the melanocytes either die or that they do not continue …
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Herstellung eines autochthonen, induzierbaren Tumormodells & Entwicklung eines PTD-Cre Induktionssystems
… fünf von zwölf doppeltransgenen Nachkommen aus Tyrosinase-floxstop-TAgxCre-del. Verpaarungen konnten nach einer Latenzzeit von neun bis elf Monaten maligne Melanome der Augen diagnostiziert werden. Dabei zeigte sich die gewebsspezifische Expression des Onkogens unter der Expressionskontrolle des …
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Induction of the retinal pigment epithelium of the chicken embryonic eye
… were investigated. Digoxigenin-labeled tyrosinase and tyrosinaserelated protein-2 (TRP-2) riboprobes were synthesised and used in ISH reactions on embryonic eye tissue. Tyrosinase transcripts were first detected at stage 19.5 (70-71 hours) and TRP-2 transcripts were detected a few hours …
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Characterisation and functional analysis of the chicken microphthalmia gene
… report of the characterisation of the chicken tyrosinase gene promoter, and only one full-length chicken microphthalmia cDNA (cmi9) has been identified to date.
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