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Showing 1 to 20 of 72 for “"Lactococcus lactis"”.
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Idlitaikinasta eristetyn Lactococcus lactis -maitohappobakteerin tuottaman bakteriosiinin karakterisointi
… kolmeen luokkaan niiden ominaisuuksien mukaan. Lactococcus-suvun bakteriosiineja on löydetty ainoastaan luokista I ja II, joihin kuuluu pieniä lämmönkestäviä peptidejä. Luokka III kattaa isommat lämpöherkät proteiinit. Bakteriosiineilla on erilaisia toimintamekanismeja nujertaa kohdebakteeri. Ne …
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Developing a vector for expressing serratiopeptidase in Lactococcus lactis
… vector that, when transformed into the probiotic Lactococcus lactis, would allow for bioactive serratiopeptidase production within the gut; thus, increasing bioavailability. The vector designed was delivered from the synthesis company in two fragments (they failed to assemble to full vector). …
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Essential Fatty Acid Biosynthetic Enzymes of Escherichia Coli and Lactococcus Lactis Subsp. Lactis
FabG, beta-ketoacyl-ACP reductase, performs the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to the beta-hydroxyacyl-ACP products. I report the first characterized fabG mutants. By chemical mutagenesis followed by a tritium suicide procedure, I obtained three conditionally-lethal …
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Mejora de la supervivencia y aptitud tecnológica de cepas industriales de Lactococcus. lactis mediante evolución adaptativa
Lactococcus lactis es el componente mayoritario de los cultivos iniciadores mesófilos utilizados en la elaboración de productos lácteos fermentados. Durante la fermentación, L. lactis se enfrenta a diferentes condiciones de estrés que, en muchas ocasiones, tienen un impacto sobre la pared celular, …
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The encapsidation protein from Lactococcus lactis asccφ28 as a novel system for the study of molecular motors
… gene product from open reading frame 11 of the Lactococcus lactis phage asccφ28, the putative encapsidation protein, has been expressed in Escherichia coli and purified to homogeneity (55.6% sequence similarity, Smith-Waterman score). Size-exclusion chromatography suggests that the recombinant …
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Technological features and ability to produce nisin by Lactococcus lactis subsp. lactis bv. diacetylactis obtained from dairy environment
Lactococcus lactis subsp. lactis bv. diacetylactis strains are used in the dairy industry to produce acetoin and diacetyl, substances that are responsible for conferring organoleptical characteristics to some fermented foods. Bacteriocin production can be an additional beneficial feature presented …
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Effet de Bifidobacterium spp. et Lactococcus Lactis ssp. cremoris sur la conservation du hareng de l'atlantique (Clupea Harengus)
… de trois traitements probiotiques ( Lactococcus lactis spp. cremoris, Bifidobacterium spp. et le mélange des deux souches (mélange Lc-B)) a été testé sur des échantillons de hareng de l'Atlantique ( Clupea harengus) afin de prolonger leur durée de conservation selon trois niveaux de …
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Identifikace bakterií mléčného kvašení v tvrdých sýrech s využitím amplifikačních metod
… na identifikaci bakterií mléčného kvašení druhu Lactococcus lactis a poddruhů Lactococcus lactis ssp. lactis a Lactococcus lactis ssp. cremoris pomocí druhově a subdruhově specifické polymerázové řetězové reakce (PCR). Metoda PCR byla použita k identifikaci bakterií druhu Lactococcus lactis v 10 …
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Röntgenstrukturanalyse der 6-Hydroxy-D-Nikotin-Oxidase aus Arthrobacter nicotinovorans sowie Darstellung und Charakterisierung einer Protein-Protein-Kontaktfläche mit der 6-Phospho-ß-Galaktosidase aus Lactococcus lactis
Die 6-Hydroxy-D-Nikotin-Oxidase (6HDNO) aus Arthrobacter nicotinovorans wurde rekombinant exprimiert, gereinigt und kristallisiert. Die Struktur wurde mit röntgenkristallographischen Methoden in drei Kristallformen bis zu einer Auflösung von 1.9 Å gelöst und verfeinert. Das Enzym katalysiert die …
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Structural and Biochemical Studies of Nisin *Biosynthesis and Autoimmunity
… produced by the Gram-positive bacteria Lactococcus lactis spp. It is first ribosomally synthesized as an inactive precursor which is then post-translationally modified into its mature biologically active form. Described herein is a two-part study investigating (1) the structural basis of …
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Development of a multi-particulate formulation of viable bacteria for oral and vaginal delivery
… of viable, genetically modified, IL-10 producing Lactococcus lactis (b) the development of a formulation for delivery of health-promoting strains of lactic acid bacteria (referred to as probiotics) to the vagina. In addition to the viability of the lactobacilli strains incorporated in the …
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Development and Optimization of a Food-Grade Antimicrobial by Lactic Acid Bacteria Isolated from Raw Milk
… performed to isolate nisin–producing Lactcoccus lactis subsp. lactis from raw milk, to evaluate their antimicrobial activity against food spoilage organisms and selected food pathogens, and to develop the conditions for improved antimicrobial production. The component of the antimicrobial …
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Activité autolytique de bactéries lactiques et action anti-escherichia coli de symbiotiques à base d'extraits d'exopolysaccharides
… coli. À l'exception du Bifidobacterium lactis et du Lactobacillus casei estraits à partir de yaourts commerciaux, tous les microorganismes provenaient du Centre de recherche des aliments de l'Université de Moncton. Les EPS on été extraits à partir de Lactococcus lactis, B. lactis et L. …
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Phylogenetic Analysis Suggests Horizontal Gene Transfer Event Within the Mitis Group Within the Genus Streptococcus Involving L-Amino Oxidase In Streptococcus Oligofermentans
… acid sequences from 19 Streptococcus species and Lactococcus lactis using pairwise between-species distances and phylogenies based on maximum likelihood (ML) suggest possible horizontal gene transfer (HGT) of L-amino oxidase (lox) conferring inhibitory properties against S. mutans to S. …
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Étude des paramètres affectant la croissance, l'équilibre et l'activité des ferments lactiques mésophiles aromatiques mixtes en contrôle de PH
… que ceux du Cheddar incluant les genres Lactococcus, Leuconostoc et Lactobacillus. Ceux-ci seraient utilisés pour la production de fromages frais. La production de ferments lactiques aromatiques complexes est traditionnellement faite sur lait et ce, sans contrôle de pH. Or, l’utilisation …
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The Staphylococcus Pseudintermedius Adhesin Spsd Contains A Central Fibronectin-Binding Domain
… confers attachment of the heterologous host <em>Lactococcus lactis</em> to fibronectin. This work has identified a centrally-located fibronectin binding domain in SpsD which binds the 30 kDa N-terminal domain of fibronectin with high affinity. The data indicate that a tandem β-zipper mechanism of …
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Optimization of Growth Conditions of Selected Exo-Polysaccharides Producing Lactic Acid Bacteria in Whey Mineral Concentrate
… (Lactobacillus helveticus ATCC 15807 and Lactococcus lactis subsp. cremoris) in whey mineral concentrate (WMC). The parameters studied were TS%, incubation temperature, overnight cooling, incubation time, pH, and the effects of added nutrients. The samples were analyzed for titratable …
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