Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes

Described herein is my thesis work towards structural and biochemical characterization of bacterial proteins involved in metabolite regulation and natural product biosynthesis. In the first case, I will describe the structural basis for transcriptional regulation of a small molecule metabolite, p-coumarate, and the remaining cases involve enzymes responsible for biosyntheses of complex natural products. Among the natural product cases are those falling under the class of ribosomally synthesized and post-translationally modified peptides (RiPPs), with a particular emphasis on the thiopeptide subclass of RiPPs. Additionally, characterization of an off-loading enzyme involved in hybrid polyketide/non-ribosomal peptide (PK-NRP) natural product biosynthesis is described.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2019

Author dc:creator

• Cogan, Dillon P.

Contributors dc:contributor

• Nair, Satish K.
• van der Donk, Wilfred A.
• Gerlt, John A.
• Rienstra, Chad M.

• biosynthesis
• natural product
• enzyme
• RiPP
• ribosomal
• peptide
• polyketide
• non-ribosomal
• DNA-binding
• thiopeptide
• lanthipeptide

Statement dc:rights

• Copyright 2019 Dillon Cogan

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/105160

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/105160