Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 92 for “"polyketide"”.
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Synthesis and Biosynthesis of Polyketide Natural Products
… have been confined to the biosynthesis of polyketides, a therapeutically and structurally diverse class of natural products, where we employed both synthetic chemistry and biochemical techniques to validate complex metabolic processes. One such example pertained to the uncertainty …
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Synthetic Studies towards Opaliferin, a Tetracyclic Polyketide Metabolite
Opaliferin (12) was originally isolated and characterised from an entomopathogenic fungus of Cordyceps sp. NBRC 106954 by the Grudniewska group in 2014. The fungus Cordyceps sp. is a single ascospore isolated from a fruiting body found on the larvae of Meimuna opalifera also known as Walker’s …
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Type III Polyketide Synthases: Discovery, Characterization, and Engineering
The polyketides are a diverse group of natural products with important applications in medicine and industry. Industry, especially the pharmaceutical industry, is under pressure to deliver "greener" chemical syntheses that are less environmentally damaging and incorporate renewable resources. There …
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Stereospecific Csp3 cross-coupling for modular polyketide synthesis
… motif that is ubiquitous amongst polyketide and other natural products. To achieve this objective, a novel class of activated beta-aryloxysilyl pinacol boronic ester nucleophiles were designed and shown to undergo highly stereospecific cross-coupling with aryl halides even when …
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Endophytes as source of polypeptide and polyketide antibiotics fengycin and bacillaene
… of antibacterial activities belongs to the polyketide antibiotics. Other cultivation parameters such as temperature and inoculum quantity were shown to have influence on the cultivation process and fengycin yield. Moreover, it was shown, that the aeration strategy during cultivation was a …
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Mangrolide A, A Novel Marine Derived Polyketide with Selective Antibiotic Activity
The ability of nature to produce complex, biologically active compounds has inspired researchers throughout history. The isolation of these molecules has been instrumental in the discovery of many medicinal compounds, especially to combat infections caused by pathogenic bacteria. Antibiotic …
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Novel Molecules from Environmental DNA-Derived Type II Polyketide Biosynthetic Gene Clusters
… microbes have been found to be rich in type II polyketide synthase (PKS) gene clusters (Kieser, Bibb et al. 2000). PKS gene clusters produce a structurally diverse collection of aromatic natural products. The nascent polyketide in these pathways is produced by a conserved minimal PKS, composed …
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Mass Spectrometric Interrogation of Thioester-Bound Intermediates on Nonribosomal Peptide and Polyketide Assembly Lines
Innovative developments in MS continue to increase the complexity of biological systems amenable for study and provide exciting research opportunities at interdisciplinary boundaries. The doctoral studies presented here have furthered the understanding of the biosynthesis of key natural products …
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Cyclization modes in type III polyketide synthases and the synthetic compounds used as probes for mechanistic studies.
Type III polyketide synthases (PKS) catalyze different cyclization reactions of linear polyketide intermediates to produce polyketides with distinct ring structures. The mechanisms of pyrone and resorcinol ring formation in type III PKSs remain unknown, despite many years of research on this family …
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Methodological Innovations in Polyketide Synthesis and their Application toward the Scalable Synthesis of Anti-Tumor Agent Spongistatin 1
… 1, their potential application to general polyketide synthesis has been demonstrated. These methodological innovations have allowed our laboratory to take tremendous strides toward the longterm goal of helping to bring a potent chemotherapeutic agent to the global market.
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Studies Towards the Discovery of Novel Natural Products Through Functional Analysis of Environmental DNA-Encoded Type II Polyketide Synthases
… natural products, synthesized by Type II polyketide synthases (PKSs) in soil-borne bacteria. Through the functional characterization of soil eDNA-derived Type II PKS containing clones, herein we attempt to identify novel PKS systems that encode structurally diverse metabolites and explore …
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STRUCTURE DETERMINATION, BIOCHEMICAL CHARACTERIZATION, AND PROTEIN ENGINEERING OF THE ACYL CARRIER PROTEIN DOMAIN FROM 6-DEOXYERYTHRONOLIDE B SYNTHASE
Polyketides are an important class of natural products. 6-Deoxyerythronolide B Synthase (DEBS) is a type 1 polyketide synthase that produces the Polyketide antibiotic drug erythromycin. DEBS is a large multi-domain enzyme complex containing multiple enzymatic domains, including acyl carrier protein …
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Dual-omics approach for 3,4-dihydroisocoumarin characterisation from Hydrangea serrata and isolation of polyketide synthases for heterologous expression in Pseudomonas putida KT2440
… superfamily, a group of plant specific Type III polyketide synthases. Chalcone synthases and their homologues, the stilbene synthases produce a huge amount of specialised plant metabolites including phenylpropanoids. The dihydroisocoumarin biosynthesis is assumed to be enhanced by elicitors and …
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Biosynthesis of Selected Antibiotics
… (9) and nargenicin (10) was established as polyketide in origin. Three metabolites previously unreported from Nocardia argetinensis var. Huang (ATCC 31306) were isolated and identified as 18-O-acetylnodusmicin (13), and 18-O-acetylnargenicin (14).
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Novel Mechanisms in Zebrafish Otolith Nucleation, Tethering, and Dysfunction
… in three zebrafish mutants with mutations in polyketide synthase, 2) otolin-1b is a putative component of the cuticular plate and involved with otolith growth and maintenance, and 3) otolin-1a, a component of the otolithic membrane, could be used to monitor risk for vestibular dysfunction …
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The design of a molecular assembly line based on biological molecules
… on the principles of the biological molecules polyketide synthase and kinesin, and in some embodiments, may employ biomolecules like DNA as components of the system. The scheme entails the construction of a polymeric chain that moves a shuttle along the chain by controlling the interactions …
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Studies on the biosynthesis of oudenone and verucopeptin
… ( 89 ) is structurally characterized by a polyketide-derived tetrahydropyranyl side chain and a 19-membered cyclodepsipeptide. A DNA fragment of 72.5 kb was isolated from a cosmid library of Actinomadura verrucosospora chromosomal DNA. Partial analysis and sequencing of this gene cluster …
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Dearomative hydroboration-enabled synthesis of idarubicinone and synthesis of minor cannabinoids and their metabolites
… representatives of tetracyclic type II polyketide natural products that are widely used in cancer chemotherapy. Although syntheses of this class of compounds have been extensively explored, all known approaches are based on annulations, relying on the union of properly pre-functionalized …
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