Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"non-ribosomal"”.
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Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes
… cases are those falling under the class of ribosomally synthesized and post-translationally modified peptides (RiPPs), with a particular emphasis on the thiopeptide subclass of RiPPs. Additionally, characterization of an off-loading enzyme involved in hybrid polyketide/non-ribosomal peptide …
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A functional genomics approach: Characterisation of two Non-ribosomal peptide synthetase genes, and investigation of an adaptive response towards alkylating DNA damage in Aspergillus fumigatus.
… encode virulence factors, or novel drug targets. Nonribosomal peptide (NRP) synthetases have been implicated in virulence of A. fumigatus and other fungi. The work presented here reports opposing roles for two previously uncharacterised NRP synthetases with respect to A. fumigatus virulence. The …
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Mechanisms of Non-Enzymatic Peptide Bond Formation: Thioester and Transamidation Routes
<p>The reaction mechanisms employed during non-ribosomal peptide (amide) synthesis in the non-ribosomal modular enzyme complex and in the enzyme glutaminyl cyclase were also used for spontaneous peptide (amide) synthesis in a buffered, abiotic solution. Analytical analyses revealed that spontaneous …
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Functional Genomic Analysis of Natural Product Biosynthesis and Secretion in Aspergillus fumigatus
… A. fumigatus produces metabolites, through a non-ribosomal peptide synthesis mechanism, including iron-chelating molecules known as siderophores and other unusual compounds such as gliotoxin. These provide the fungus with a unique strategy for survival in the host, and contribute to …
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Untersuchungen auf Sekundärstoffproduktion und physiologische Charakterisierung von marinen heterotrophen Bakterien aus dem deutschen Wattenmeer
… for polyketide synthase-, FADH2-halogenase- and non-ribosomal peptide synthetase genes. Additionally agar diffusion assays were applied to investigate the antagonistic activity of the strains, and some bacteria were tested for acylated homoserine lactones (AHL) production. Three isolates were …
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Screening environmental actinobacteria for antimycobacterial antibiotics and characterisation of Kribbella stellenboschensis sp. nov
… isolates to produce ansamycin, glycopeptide, non-ribosomal peptide, and/or TypeII polyketide antibiotics was determined by detection of antibiotic biosynthetic gene clusters using PCR. Strain M27 demonstrated the potential to produce all the aforementioned antibiotics. Strain Y10 demonstrated …
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Detection and Purification of a Novel Natural Inhibitory Compound from an Isolated Strain of <em>Rhodococcus</em> Using an Agar Extraction Method
… indicates the presence of 24 non-ribosomal peptide synthases and 7 polyketide synthases possibly involved in production of secondary metabolites. The use of a solid agar extraction method to screen soil isolates of <em>Rhodococcus</em> for compounds with inhibitory activity …
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Genomics and transcriptomics of the industrial acarbose producer Actinoplanes sp. SE50/110
… biosynthetic gene cluster sequence, several new non-ribosomal peptide synthetase-, polyketide synthase and hybrid-clusters were identified on the Actinoplanes genome. Another key finding represents the discovery of a functional actinomycete integrative and conjugative element, which might pose an …
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Biosynthetic characterisation of secondary metabolites from stromatolite cyanobacteria Symploca sp. 1013
… to the presence of polyketide synthase (PKS) or non-ribosomal peptide synthetise (NRPS). Bioactivities in areas such as antibacterial, antifungal and anticancer have been implicated in many of these metabolites. In the present study, various marine cyanobacterial isolates from from stromatolites …
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Studies on the biosynthesis of oudenone and verucopeptin
The biosynthesis of the fungal metabolite oudenone ( 67 ) was investigated. The Ì-diketone ( 70 ) was synthesized and shown to be the open-chain biosynthetic precursor of oudenone. Intact incorporation of 70 into 67 was achieved upon incubation of 2 H-labeled, N -acetylcysteamine thioester …
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The role of genomic islands in virulence of Pectobacterium carotovorum subspecies brasiliensis on potatoes
… disease of potatoes were compared to those of non-blackleg causing strains, to identify GIs in blackleg causing strains and novel virulence factors encoded on these islands. First, the identity of a highly aggressive P. carotovorum subsp. carotovorum strain ICMP19477 and other New Zealand …
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Nla28 Activator Modulates σ54-dependent Gene Regulation in Myxococcus xanthus
… metabolism, it synthesizes polyketide (PK) and non-ribosomal peptide (NRP) natural products that are crucial sources of antibiotics. Bacteria including M. xanthus, require the alternative σ54 regulatory system to initiate the transcription of necessary genes for settling stress and completing …
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Identifying novel proteins in translation complex by using analytical ultracentrifugation with fluorescent detection system
… spectrometric studies, we have identified 41 non-ribosomal proteins and non-translation initiation factors as possible components of the translation initiation complex. To determine which of these proteins are in the translation complex, we applied analytical ultracentrifugation with …
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Characterizing and Engineering Thioesterases as Biocatalysts for Macrocyclization
… here describes the characterization of two non-ribosomal peptide synthetase (NRPS) TE domains and their use as biocatalysts for the chemoenzymatic synthesis of two cyclic depsipeptide NP. Unlike other characterized NRPS TEs, these two demonstrated the ability to catalyze macrolactonization …
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Characterising the response to gliotoxin exposure in Aspergillus fumigatus ΔgliT and ΔgliZ
The non-ribosomal peptide gliotoxin, is a toxic fungal secondary metabolite produced by the filamentous fungus Aspergillus fumigatus. Previous work has implicated its potent anti-fungal properties and importance as a virulence factor in human infection. Availability of the A. fumigatus genome has …
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Functional Characterisation of the Eukaryotic Translation Elongation Factor 1Bγ in Aspergillus fumigatus; A Proteomic Systems Approach
… comparative proteomics of A. fumigatus Δpes3, a non-ribosomal peptide synthetase deficient strain, supports a structural role for this peptide with the altered expression of proteins functioning in morphogenesis and germination. A. fumigatus Δpes3 exhibited a reduced germination rate in static …
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The post-translational methylation of arginine in the glycine arginine rich region of CHO nucleoin
… the in vivo interactions of nucleolin with other non-ribosomal nucleolar proteins. In support of previous work, DGAR caused endogenous B23 to redistribute to the nucleoplasm, suggesting an in vivo interaction. Endogenous Nopp140 shows no redistribution in the presence of DGAR. The effect of DGAR …
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Characterisation of microbial Mat communities in meltwater ponds of the McMurdo ice shelf, Antarctica
… producer could not been identified. However non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) genes were identified which could be the genetic basis for novel bioactives. The use of a multi-disciplinary approach synthesis and subsequent results significantly increased …
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Studies of new natural products and their biosynthesis spearheaded by high-resolution mass spectrometry
… immunosuppressant activity are synthesized by non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). In NRPS and PKS biosynthesis, monomer units are incorporated into a growing natural product chain in an assembly line fashion using a covalent thiotemplate mechanism. Because …
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Identification and characterization of ice nucleation active bacteria isolated from precipitation
… These two isolates represent the first confirmed non-gammaproteobacteria with INA. After further characterization, the two isolates of Lysinibacillus did not appear to use a protein to freeze water. Instead, the Lysinibacillus isolates used a secreted, nanometer-sized molecule that is heat, …
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