EXPRESSION AND PROCESSING OF HIV-1 GP160 WITH HOMOLOGOUS AND HETEROLOGOUS SIGNAL SEQUENCES USING A VSV VECTOR SYSTEM

Previous research has shown that when the natural signal sequence (NSS) of HIV 1 gpl20 was substituted with the honeybee mellitin signal sequence (MSS), the gpl20 became efficiently secreted. However, this work was carried out using the baculovirus expression system in which the processing of gpl20 is not identical to that in mammalian cells. Therefore, I expressed HIV-1 gpl60 with different signal sequences using the Vesicular Stomatitis Virus New Jersey serotype (VSVnj) vector system. I found that the HIV-1 gpl60 protein can be efficiently expressed and processed when the HIV-1 gpl60 gene was inserted between the G and L genes of VSVNJ. The processing of the gpl60 protein and the release of gpl20 was more efficient with MSS than with the NSS. This result verified that the natural signal sequence of HIV-1 gpl60 plays an important role in the inefficient processing of gpl60

Name thesis:degree_name

M Sc

Discipline thesis:degree_discipline

Microbiology and Immunology

Year dc:date.issued

2006

Author dc:creator

• Wu, Kunyu

Advisor dc:contributor.advisor

• Kang, C. Yong

• VSV
• Gene expression vector
• Reverse genetics
• HIV-1
• Env
• Signal sequence

Handle dc:identifier.uri

https://hdl.handle.net/20.500.14721/20423

OAI identifier oai:identifier

oai:uwo.scholaris.ca:20.500.14721/20423