Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 220 for “"Env"”.
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Dissociating Siv Env and Cd4: Consequenes for Virus and Host
… we found that a mutation in the V1/V2 loops of Env was required for CD4-independent entry, and that acquisition of CD4-independence resulted in an increase in neutralization sensitivity. While iMac239 was CD4-independent, its CD4-binding site was intact, thus we removed the Aspartic Acid residue …
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Präklinische Evaluation einer Vakzinierungsstrategie gegen HIV-1 basierend auf konformationellen Epitopen des Env-Glykoproteins
… Immunantwort, welches auf konformationellen Env-Epitopen basiert, die durch die Interaktion mit dem zellulären Rezeptorkomplex (CD4/CXCR-4) induziert werden. In unserer Arbeitsgruppe wurde bereits gezeigt, dass Pseudovirionen (PV) mit eingebauten HIV-Env- bzw. CD4/CXCR-4-Molekülen eine …
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Die Rolle des C-Terminus von Env in der HIV-1-Infektion und -Replikation
… des ungewöhnlich langen C-Terminus von HIV-1-Env (Env-CT) in der Infektion und Replikation zu gewinnen. Es ist bisher nicht bekannt, aus welchen Gründen die Länge des Env-CTs in vivo streng konserviert ist und Virusmutanten mit trunkierten Env-CTs in vitro einen Replikationsblock in …
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Conformational engineering of human chemokine receptors and HIV-1 Env using deep mutational scanning
… and binding to the HIV-1 surface glycoprotein Env. Using these landscapes, I identified that the chemokine CXCL12 binds asymmetrically in the CXCR4 ligand-binding cavity, discovered mutations within a potential allosteric site in CXCR4 that enhance CXCL12 binding, and engineered a CCR5 variant …
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Investigation of particulate HIV-1 Env vaccine candidates using Zera® and SpyTag/SpyCatcher technologies
The HIV-1 envelope glycoprotein (Env) is the primary focus of prophylactic HIV vaccine development. However, the unusually low density of Env spikes on the virion (≈14 spikes/virion) is unfavourable for eliciting high titre, long-lasting antibody responses. It is possible that increasing the Env …
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Characterization of The Role(S) of Env Protein of Human Endogenous Retrovirus-K In Multiple Human Cancers
… Our laboratory reported that anti-HERV-K envelope (Env) protein antibodies show antitumor potential in targeting breast tumors, indicating that HERV-K Env (Kenv) may play an important role in tumorigenesis. In this study, we used either knockdown (main focus) or over-expression approach, …
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Generation and characterization of HIV-1 subtype C candidate vaccines that will induce high titre antibody responses to HIV-1 envelope glycoprotein
… Africa. Specifically, this involved use of the envelope glycoprotein sequence of the CAP256 superinfecting virus (CAP256_SU) from the CAPRISA 002 cohort, and a mosaic Gag sequence which resulted in robust autologous Tier 2 neutralisation of CAP256_SU pseudovirions. The envelope glycoprotein …
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Charakterisierung der Prototyp Foamyvirus Hüllglykoprotein Rezeptorbindungsdomäne
… Das Partikel-assoziierte Hüllglykoprotein (Env-Protein) des „Prototype Foamy Virus“ (PFV) ist im Vergleich zu anderen retroviralen Hüllglykoproteinen einzigartig. Die Koexpression des PFV Env-Proteins für die PFV-Partikelfreisetzung ist essenziell und die spezifische Funktion kann nicht von …
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A Biophysical Investigation of HIV-1 Envelope Cytoplasmic-Tail Mediated Retention in the Gag Lattice
… successful incorporation of the trimeric viral envelope glycoprotein, Envelope (Env), into Gag lattice assembly sites on the host-cell plasma membrane during virus biogenesis. The mechanistic nature of interaction between Gag, the structural protein, and Env, the viral spike, are dependent on …
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Characterisation of HIV-1 subtype C envelope functional determinants of dual infected individuals
Identification of HIV-1 Envelope (Env) fitness determinants could provide functionally constrained, accessible regions that could be included in subunit vaccines to induce broadly neutralising antibodies (bnAb). We hypothesised that Env fitness determinants are common to circulating variants but …
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The Design And Synthesis Of Inhibitors Of Hiv-1 Viral Entry And Photoaffinity Labeled Probes For Structural Elucidation Of The Hiv-1 Env
… these compounds increase the ability of the Env trimer to sample a more open conformation which sensitizes the Env to antibody mediated neutralization and elimination (ADCC). Secondly, HIV-1 infection inhibition and lysis of HIV-1 virions has been accomplished via the design, synthesis and …
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From Fossils to Function: Exploring the Evolutionary And Functional Diversity Of HERV Envelope Proteins Across Primate Lineages
… key protein-coding genes: gag, pro, pol, and env, flanked by long terminal repeats (LTRs). The env gene, responsible for virus entry into cells, has evolved under various selection pressures, leading to its significant role in the study of diseases like cancer, autoimmunity, neurodegenerative …
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To compare the expression, processing, incorporation and function of pseudoviruses and infectious molecular clones using different cell types and HIV backbones
… This study therefore aimed to determine whether Envelope (Env) expression and processing affects function and whether cell type and/or expression system were responsible for these differences. Our data suggest that even though we did not observe differential expression of recombinant Env clones …
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Isolation of Moloney Leukemia Virus Temperature Sensitive Mutants, and the Characterization of a Temperature Sensitive Mutant Deficient in the Incorporation of Env Gene Proteins
Techniques for the isolation of temperature sensitive (ts) mutants and revertants of Moloney leukemia virus (M-MuLV) and analysis of ts 7, an M-MuLV ts mutant isolated by Dr. P. K. Y. Wong are presented in this dissertation.
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The impact of HIV-1 subtype C Envelope N-glycosylation on DC-SIGN meditated modulation of DC function to facilitate transmission or enhance viral pathogenesis
N-glycosylation plays an important role in Envelope (Env) function and may be involved in the modulation of the immune response to HIV-1 infection. In this study, we hypothesized that Env N-glycosylation may affect viral pathogenesis by influencing Env structure and function. Furthermore, we also …
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Untersuchungen zum Transport und Abbau des HIV-1 Envelope Glykoproteins
Die Expression des HIV-1 Env Glykoproteins wird von verschiedenen Faktoren beeinflußt, die sowohl auf die Env mRNA als auch auf das Glykoprotein wirken. Diese Faktoren verursachen eine sehr effiziente Suppression des Glykoproteins, weshalb nur ein geringer Anteil von Env auf der Zelloberfläche …
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Targeting An Ancient Retrovirus During Melanoma Using Adoptive T Cells Therapy
… active variants of HERV, such as the envelope (env) protein of HERV-K, are expressed on the surface of melanoma, but not normal cells. To target HERV-K, T cells were engineered to express a CAR specific for this env protein, by replacing the antigen-binding exodomain of CD19-specific …
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The role of HIV-1 subtype B Envelope transmission motifs in subtype C variant infectivity
… subtype B TF and mismatched chronic infection envelope (env) sequences and identified two putative transmission motifs: Histidine at position 12 of the signal peptide (His12) and a potential N-glycan site (PNG) at position 413-415. Although, His12 was shown to be important for subtype B Env …
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