Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"Signal sequence"”.
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EXPRESSION AND PROCESSING OF HIV-1 GP160 WITH HOMOLOGOUS AND HETEROLOGOUS SIGNAL SEQUENCES USING A VSV VECTOR SYSTEM
Previous research has shown that when the natural signal sequence (NSS) of HIV 1 gpl20 was substituted with the honeybee mellitin signal sequence (MSS), the gpl20 became efficiently secreted. However, this work was carried out using the baculovirus expression system in which the processing of gpl20 …
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Engineering and characterisation of anti-progesterone OBodies
… the OBody. During phage display selection a new signal sequence was fortuitously discovered that provided an advantage during phage display. The new signal sequence was investigated and characterised using mutants of GFP and a DARPin sequence to uncover the nature of the selective advantage. The …
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[alpha](1,3)galactosyltransferase mediated gene therapy for cancer: developing new models and improving on existing ones
… phospholipase C (PIPLC). The PIPLC native signal sequence was replaced with the human epidermal growth factor signal sequence, EGFssPIPLC, to induce secretion from mammalian cells. (Abstract shortened by UMI.)</p>
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Secretion of active recombinant phytase from stably transformed soybean cells
… or without a patatin endoplasmic reticulum (ER) signal sequence. All four vectors were sequenced and introduced into 'Williams 82' suspension culture cells by particle bombardment. Stably transformed cell lines were selected and tested for stable integration by Southern analysis. The presence of …
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Beyond MICS: Determining the Role of Porin Production and β-Lactamase Expression in Resistance and Cellular Physiology of Klebsiella pneumoniae
… Cpx, and σE regulon. Removal of the CTX-M-15 signal sequence altered the transcriptomic profile and led to upregulation of cytoplasmic chaperones but did not fully ameliorate the effect of the β-lactamase. Biofilm formation was decreased when CTX-M-15 was expressed, in a protein signal …
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Development of Brucella abortus RB51 as a Vaccine to Protect Against Brucellosis and Anthrax
… Brucella. The PA4 domain was fused to Brucella signal sequences of Brucella 18kDa protein, superoxide dismutase or no signal sequence to localize the PA4 to the outside cell envelope, periplasmic space or cytosol respectively. Comparisons of the expression level and stability of the native and …
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Investigation of conventional and unconventional protein secretion using galectin-3
… is well known, and involves proteins with a signal sequence being directed into the endoplasmic reticulum (ER). Once in the lumen of the ER, proteins are trafficked through the Golgi and remain separate from the cytosol throughout their journey to the outside of the cell. More recently, …
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Incorporation of tumor antigen epitopes into cytokine signal peptides enhance the efficacy of immuno-gene therapy
… expression of antigen epitopes within cytokine signal peptides. We first investigated the possibility of modifying cytokine signal peptides with antigen epitopes without aborting function. We inserted the genes encoding the MHC class I restricted antigenic epitopes of chicken ovalbumin (OVA), …
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The Utilization of the Hmg2 Inducible Promoter to Genetically Engineer Parasite Resistance in Tobacco
… region of the tomato PI-I gene, lacking the signal sequence (EM1), or the coding region of PI-I including the signal sequence (EM2), fused to the nematode-responsive Hmg2 promoter. Transgenic PI-I plants were inoculated with G. t. tabacum cysts and evaluated for nematode interactions. Our …
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Exploration of the molecular structure of Escherichia coli cytochrome bo ubiquinol oxidase by genetic approach
… cyoA and cyoB and one base in the overlapping sequence between cyoB and cyoC, in-frame fusions are made between all three subunits (II-I-III), the resulting gene product still assembles as part of a functional oxidase. The fused subunit (II-I-III) contains 22 transmembrane spans. These data …
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New hybrid automatic repeat request (HARQ) combining with space time block coding (STBC) in invariant and variant fading channel
… channel. With MA-STBC, there exist different signal packets alternatives for retransmission that can better exploit the characteristics of the channel. An algorithm that chooses the best signal sequence alternatives for retransmission and which exploit better the characteristics of the current …
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Development of an in-cell förster resonance energy transfer technique to study protein structure inside living cells
… to its intracellular destiny based on its signal sequence. This will result in special mammalian cells that contain a fluorescence labeled target protein with unlabeled intracellular endogenous proteins as the background. The FRET measurement will be performed on this specifically labeled …
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Structure, membrane association, and processing of meprin subunits
… genes. The β subunit cDNA was cloned and sequenced from mouse kidneys, and the membrane association and the in vivo proteolytic processing of mouse meprin subunits were investigated. The primary translation product of the meprin β subunit is composed of 704 amino acids, and contains …
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RNA Localization and Translational Regulation on the Endoplasmic Reticulum
… cell fate specification, and synapse formation. Signal sequence-encoding mRNAs are localized to the endoplasmic reticulum (ER) membrane by either of two mechanisms, a canonical mechanism of translation on ER-bound ribosomes (signal recognition particle pathway), or a poorly understood direct ER …
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Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris
… protein, and fused to the S. cerevisiae MATa signal sequence. Expression plasmids were successfully transformed in P. pastoris strain yGS 115 followed by induction. Western blot analyses confirm the expression of β-galactosidase and colorimetric activity assays further validate enzymatic …
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Characterization of the expression profile of polyamine biosynthetic genes (Spermidine synthase) and polyamine metabolic regulation in arabidopsis
… 13-glucuronidase protein by attaching PEST signal sequence from mouse ornithine decarboxylase at N or/and C terminus. Modified proteins were shown to have variable reduction in their stability in Arabidopsis seedlings and cell cultures. This should open up a way to use GUS as a more …
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Cloning, High-Level Expression, and Characterization of Escherichia Coli Cytochrome B(562)
… length gene, including the promotor. The DNA sequence analysis of the gene revealed the presence of a leader sequence, with the characteristics of an E. coli signal sequence, in front of the coding sequences for the mature protein. Localization studies on the overexpressed protein revealed …
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Biochemical And Structural Characterization Of The Core Subunits Of Gpi Transamidase
… of various proteins that contain a proper GPI-T signal sequence. Gpi8, Gaa1, Gpi16, Gpi17 and Gab1 are the five known subunits that may encompass the fungal GPI-T; Gpi8 is the catalytic subunit, but the functions of the other subunits remain essentially unknown. In humans, different GPI-T …
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Mechanisms of Cell Surface Trafficking and Potential Functions of Extracellular Annexin A2
… remains unknown. Since, AnxA2 does not possess a signal sequence that is essential for the classical ER-Golgi secretion; we believe that the protein follows a non-classical pathway of protein secretion. Here, we show that Ca2+- induced translocation of AnxA2 to the cell surface is a multi-step …
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Cloning, sequencing and physicochemical characterization of a hemagglutinin from Escherichia coli 09:H10:K99
… unambiguously by subcloning. The nucleotide sequence of the gene was determined and found to consist of a 792 base pair open reading frame coding for a protein of 29 kDa. This protein has a predicted prokaryotic N-terminal secretory signal sequence. The protein sequence derived from the …
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