Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters

Ribosomally synthesized and post-translationally modified peptides are an abundant source of bioactive natural products. Using genome-mining techniques, new hybrid biosynthetic gene clusters have been discovered which contain the genes necessary for both lanthipeptide formation as well as modification on the precursor peptides by a radical SAM enzyme. Two examples of this type of gene cluster that are of particular interest to us are one from Scytonema hofmannii PCC 7110 and another from Butyrivibrio sp. VCD2006. Bioinformatic analyses were performed on these clusters, and they were coexpressed using the FAST-RiPPs method to characterize the modifications on the precursor peptides. The final natural products from these clusters could potentially have interesting bioactivities.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2023

Author dc:creator

• Frerk, Autumn M.

Contributors dc:contributor

• van der Donk, Wilfred A

• Ripp
• Lanthipeptide
• Radical Sam
• Biosynthetic Gene Clusters
• Fast-ripps

Statement dc:rights

• Copyright 2023 Autumn Frerk

Language dc:language

en, eng

Handle dc:identifier

https://hdl.handle.net/2142/122000

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F122000