Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Ripp"”.
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Post-translational modifications catalyzed by RiPP and RiPP-adjacent enzymes
… and post-translationally modified peptides (RiPPs) are, as their name suggests, constructed from ribosomal peptides. The scope of biosynthetic reactions utilized by Nature to transform these peptides into structurally fascinating natural products is astounding. Leveraging these enzymes for …
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Expansion of predicted RiPP biosynthetic sequence space using the RiPP recognition element
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… and post-translationally modified peptides (RiPPs) use enzymatic chemistry to diversify simple peptide backbones into architecturally complex and functionally rich natural products. These dedicated peptide-modifying enzymes install heterocycles, macrocycles, acyl groups, halogens, and other …
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Genomics-Guided Discovery and Characterization of Ancillary RiPP Modifications
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01
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Elucidation and control of substrate recognition during RiPP biosynthesis
… and posttranslationally modified peptides (RiPPs) are a rapidly growing class of natural products. RiPP precursor peptides can undergo extensive enzymatic tailoring to yield structurally and functionally diverse products. Cyclodehydratases are a type of RiPP modifying enzyme that catalyze …
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Investigation of radical SAM-mediated thioether installation in RiPP natural products
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-05-01
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Expanding the structural diversity of the RiPP class of natural products
… and post-translationally modified peptides (RiPPs) are a diverse and rapidly expanding class of natural products. Despite starting out as linear chains of amino acids, RiPP natural products acquire structural diversity ranging from small molecules like microcin C7 to 48-mer polytheonamides …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… and Posttranslationally modified Peptides (RiPPs) are a particularly intriguing class. These compounds begin as a genetically encoded precursor, which is translated into a short (~50) residue peptide. This precursor peptide is then modified by genetically co-located enzymes to generate a …
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Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms
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Investigating and engineering the post-translational modifications in RiPP natural product biosynthesis (I) Thiopeptides (II) Sactipeptides
… and post-translationally modified peptides (RiPPs) have been attracting interest as one such source of untapped potential. Owing to the unique “promiscuous-yet-specific” biosynthetic paradigm that RiPPs offer, this class of natural products have been an area of intense research. Chapter 1 …
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Characterization of Cyclopropyl Synthases Involved in the Maturation of Ribosomally Synthesized and Posttranslationally Modified Peptides
… and post-translationally modified peptides (RiPPs) are a large class of natural products with significant human health implications. RiPPs are synthesized from a genetically encoded precursor peptide that undergoes significant modifications by maturing enzymes, or maturases. Recently, …
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Biosynthetic and bioinformatic investigation of RiPPs with radical SAM-installed crosslinks
… and post translationally modified peptides (RiPPs) are an expanding class of natural products containing new chemical modalities and bioactive molecules. Although with diverse biological functions, significant research has been focused on investigating RiPPs with antibacterial activity due to …
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Biochemical and structural studies on proteins involved in the production of natural products and degradation of polysaccharides
… of proteins involved in the biosynthesis of RiPP natural products and polysaccharide degradation. RiPP (ribosomally synthesized and posttranslationally modified peptide) natural products represent a growing class of compounds. They are biosynthesized from a linear precursor peptide that was …
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Discovery and characterization of ribosomally synthesized and post-translationally modified peptide natural products
… and post-translationally modified peptide (RiPP) natural products are genetically encoded peptide-derived secondary metabolites whose structures can be predicted based on gene sequence and identity of tailoring enzymes within the biosynthetic gene cluster. Post-translational modifications …
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Engineering of the microviridin post-translational modification enzymes for the production of synthetic protease inhibitors
… and post-translationally modified peptides (RiPPs) are a group of natural products generated by the action of post-translationally modifying enzymes on precursor peptides translated from mRNA by ribosomes. The great substrate promiscuity exhibited by many of the enzymes from RiPP biosynthetic …
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Competition-based CRISPR-dCas9 transcriptional control mechanisms and application of dCas9 biosensors for high-throughput, cell-based protease inhibitor screens
… as an in vivo biosensor, I integrate it with a RiPP-based molecular library and an in vivo selection system to screen for inhibitors of the SARS-CoV-2 Papain-like protease (PLpro). With this integrated system, I screened tens of millions of RiPPs and identified DAA680, a 13-AA modified peptide …
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New Insights into Mycofactocin Biosynthesis, Structure and Function
… and post-translationally modified peptide (RiPP)-derived redox cofactor. Its biosynthesis is accomplished through the dedicated actions of the products of six conserved genes, <em>mftABCDEF</em>. The mycofactocin pathway is one of the most widely distributed RiPP systems in bacteria however, …
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Studies on the mechanism of action of the antimicrobial S-linked glycopeptide sublancin
… and post-translationally modified peptide (RiPP) natural products are a rapidly expanding class of compounds with antimicrobial activity. Sublancin is one of five members of the glycocin family of RiPPs, and contains an unusual S-linked glycosylation. This unprecedented post-translational …
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Mechanistic Insights into MftR-Dependent Regulation of the Redox Cofactor Mycofactocin
… and posttranslationally modified peptides (RiPPs). To achieve their mature form, the genetically encoded RiPP precursor peptide undergoes significant post-translational modifications by diverse families of tailoring enzymes. Following synthesis by the ribosome, modifying enzymes process the …
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Development Towards an Ethylene Sensing Artificial Metalloenzyme
… and post-translationally modified peptide (RiPP) hypothesized to function as a redox cofactor in bacteria, particularly the Mycobacteria genus, which includes M. tuberculosis. In order to further elucidate the function of MFT and its associated proteins, a synthetic route was desired to the …
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