{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/122000"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/122000","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_has_math":false,"creators":["Frerk, Autumn M."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["van der Donk, Wilfred A"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12","date_published":"2023-12","updated_at":"2026-07-22T22:25:00Z","subjects":["Ripp","Lanthipeptide","Radical Sam","Biosynthetic Gene Clusters","Fast-ripps"],"languages":["en","eng"],"rights":["Copyright 2023 Autumn Frerk"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/122000","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["van der Donk, Wilfred A"]},{"key":"dc:creator","label":"Author","values":["Frerk, Autumn M."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-12","2023-11-20"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ripp","Lanthipeptide","Radical Sam","Biosynthetic Gene Clusters","Fast-ripps"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Autumn Frerk"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/122000"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Autumn Frerk, accepted the attached license on 2023-11-19 at 12:18.","The student, Autumn Frerk, submitted this Thesis for approval on 2023-11-19 at 12:25.","This Thesis was approved for publication on 2023-11-20 at 09:15.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19961 on 2024-03-01 at 13:14:48","Ribosomally synthesized and post-translationally modified peptides are an abundant source of bioactive natural products. Using genome-mining techniques, new hybrid biosynthetic gene clusters have been discovered which contain the genes necessary for both lanthipeptide formation as well as modification on the precursor peptides by a radical SAM enzyme. Two examples of this type of gene cluster that are of particular interest to us are one from Scytonema hofmannii PCC 7110 and another from Butyrivibrio sp. VCD2006. Bioinformatic analyses were performed on these clusters, and they were coexpressed using the FAST-RiPPs method to characterize the modifications on the precursor peptides. The final natural products from these clusters could potentially have interesting bioactivities."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters"]}]}],"canonical_facts":{"dc:contributor":["van der Donk, Wilfred A"],"dc:creator":["Frerk, Autumn M."],"dc:date":["2023-12","2023-11-20"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Autumn Frerk, accepted the attached license on 2023-11-19 at 12:18.","The student, Autumn Frerk, submitted this Thesis for approval on 2023-11-19 at 12:25.","This Thesis was approved for publication on 2023-11-20 at 09:15.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19961 on 2024-03-01 at 13:14:48","Ribosomally synthesized and post-translationally modified peptides are an abundant source of bioactive natural products. Using genome-mining techniques, new hybrid biosynthetic gene clusters have been discovered which contain the genes necessary for both lanthipeptide formation as well as modification on the precursor peptides by a radical SAM enzyme. Two examples of this type of gene cluster that are of particular interest to us are one from Scytonema hofmannii PCC 7110 and another from Butyrivibrio sp. VCD2006. Bioinformatic analyses were performed on these clusters, and they were coexpressed using the FAST-RiPPs method to characterize the modifications on the precursor peptides. The final natural products from these clusters could potentially have interesting bioactivities."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/122000"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Autumn Frerk"],"dc:subject":["Ripp","Lanthipeptide","Radical Sam","Biosynthetic Gene Clusters","Fast-ripps"],"dc:title":["Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}