Multi-omic profiling of NPQ improved nicotiana tabacum lines under uniform light conditions

Non-photochemical quenching (NPQ) is an essential process protecting plants from oxidative damage under high light conditions. During the transition from light to shade, photosynthesis is briefly limited based on the rate of NPQ relaxation. Increasing expression of NPQ mediating proteins (VDE, ZEP, & PSBS) using an Arabidopsis T-DNA cassette in Nicotiana tabacum L. (tobacco) has been shown to improve the rate of NPQ relaxation, resulting in yield increases under both greenhouse and field conditions (Kromdijk et al., 2016). However, the pleiotropic effects of this gene manipulation have yet to be examined. To address this a comparative, multi-omics approach, two previously described transgenic tobacco lines (VPZ23 & VPZ34) with increased rates of NPQ relaxation were analyzed. The results indicate minimal, but directed, changes to the transformants’ transcriptome and proteome centered that could lower plant biotic defenses.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Plant Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2021

Author dc:creator

• Hutchinson, Sarahann M.

Contributors dc:contributor

• Long, Stephen P

• Non-photochemical quenching
• photosynthesis
• proteomics
• transcriptomics
• lipidomics
• oxylipins

Statement dc:rights

• Copyright 2021 Sarahann Hutchinson

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/110775

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/110775