High-throughput screening for ADP-ribosyl hydrolase 3 inhibitors

Poly(ADP-ribose) (PAR) signaling is an important component of DNA damage repair, DNA replication, and initiation of parthanatos, a method of controlled cell death. While PAR has been well studied for decades, much is still unknown about PAR’s initiation of parthanatos and the importance of mono(ADP-ribose) (MAR) in cellular regulation. The lack of knowledge in this field can be attributed in part to the lack of a cell-permeable tool compound to study the activity of ARH3, a key degrader of PAR and MAR. Toward filling this need, a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2021

Author dc:creator

• Farmer, Rachel Elizabeth

Contributors dc:contributor

• Hergenrother, Paul J.

• ARH3
• PAR
• Screen

Statement dc:rights

• Copyright 2020 Rachel Farmer

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/109637

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/109637