{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/109637"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/109637","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"High-throughput screening for ADP-ribosyl hydrolase 3 inhibitors","abstract":"Poly(ADP-ribose) (PAR) signaling is an important component of DNA damage repair, DNA replication, and initiation of parthanatos, a method of controlled cell death. While PAR has been well studied for decades, much is still unknown about PAR’s initiation of parthanatos and the importance of mono(ADP-ribose) (MAR) in cellular regulation. The lack of knowledge in this field can be attributed in part to the lack of a cell-permeable tool compound to study the activity of ARH3, a key degrader of PAR and MAR. Toward filling this need, a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3.","abstract_html":"Poly(ADP-ribose) (PAR) signaling is an important component of DNA damage repair, DNA replication, and initiation of parthanatos, a method of controlled cell death. While PAR has been well studied for decades, much is still unknown about PAR’s initiation of parthanatos and the importance of mono(ADP-ribose) (MAR) in cellular regulation. The lack of knowledge in this field can be attributed in part to the lack of a cell-permeable tool compound to study the activity of ARH3, a key degrader of PAR and MAR. Toward filling this need, a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3.","abstract_has_math":false,"creators":["Farmer, Rachel Elizabeth"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Hergenrother, Paul J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-03-05T21:47:34Z","date_published":"2021-03-05T21:47:34Z","updated_at":"2026-07-22T22:24:50Z","subjects":["ARH3","PAR","Screen"],"languages":["en"],"rights":["Copyright 2020 Rachel Farmer"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/109637","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hergenrother, Paul J."]},{"key":"dc:creator","label":"Author","values":["Farmer, Rachel Elizabeth"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-03-05T21:47:34Z","2023-03-05T21:47:41Z","2020-12-08","2020-12"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ARH3","PAR","Screen"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2020 Rachel Farmer"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/109637"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Poly(ADP-ribose) (PAR) signaling is an important component of DNA damage repair, DNA replication, and initiation of parthanatos, a method of controlled cell death. 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While PAR has been well studied for decades, much is still unknown about PAR’s initiation of parthanatos and the importance of mono(ADP-ribose) (MAR) in cellular regulation. The lack of knowledge in this field can be attributed in part to the lack of a cell-permeable tool compound to study the activity of ARH3, a key degrader of PAR and MAR. Toward filling this need, a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. 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