Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast

In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1⁺ was tagged at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4⁺. We observed that 20% of the cells were undergoing division. Next we observed the effects that deleting ben1⁺ would have on the functions of Mto1p and Mto2p. We setup crosses between these strains and observed their localization. We did not observe any specific Mto2-GFP localization in ben1Δ. cells. This suggests that Ben1p plays a role in the proper localization of a γ-TuC associated protein.

Name thesis:degree_name

Master of Science (M.S.)

Level thesis:degree_level

Thesis - Pacific Access Restricted

Discipline thesis:degree_discipline

Biological Sciences

Year dc:date.available

2009

Author dc:creator

• Escotto, Benjamin Alan

Contributors dc:contributor

• Srinivas Venkatram

• Microtubules
• Microtubules Cloning
• Fungal proteins
• Mutagenesis
• Biology
• Life Sciences

Licence

• http://rightsstatements.org/vocab/InC/1.0/

Repository record dc:identifier

https://scholarlycommons.pacific.edu/uop_etds/715

OAI identifier oai:identifier

oai:scholarlycommons.pacific.edu:uop_etds-1714