Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"intracellular localization"”.
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Intracellular localization, biochemical and biophysical properties of human Armet
… staining was used to verify Armet’s localization in ER and Golgi apparatus in MBA-MD-231 cells. Evidence for calcium binding by Armet was obtained by circular dichroism spectroscopy (the binding of calcium appeared to decrease helix content), by differential scanning calorimetry …
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Exploring protein interactions and intracellular localization in regulating flavonoid metabolism
… protein-protein interactions and the subcellular localization of enzymes is believed to be fundamental to many aspects of metabolism. Although this organization has been demonstrated in several systems, the mechanisms by which it is established and regulated are still not well understood. The …
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Apoptosis and porphyrin biosynthesis by a photodynamic chemotherapeutic
… Subsequent exposure to light in vitro causes intracellular Proto to initiate formation of singlet oxygen molecules, leading to self-destruction. This photo-activated destruction by ALA is enhanced by addition of the tetrapyrrole modulator 1,10-phenanthroline (Oph), by enhancing Proto …
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Studying Tissue Specific Diversity of Splicing Regulator Muscleblind
… and abdominal muscles showed high Mbl levels. Intracellular localization of Mbl was typically nuclear, however in the nervous system the protein was strongly expressed in the cytoplasm. During early adult development in the pupa, in various tissues Mbl was initially detected in discrete nuclear …
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A Monte Carlo-based Model Of Gold Nanoparticle Radiosensitization
… cell line, and AuNP size, concentration and intracellular localization. This thesis explored the physics and some of the underlying mechanisms behind AuNP radiosensitization. A Monte Carlo simulation approach was developed to investigate the enhanced photoelectric absorption within AuNPs, and …
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E7 PROTEINS OF HIGH-RISK (TYPE 16) AND LOW-RISK (TYPE 6) HUMAN PAPILLOMAVIRUSES REGULATE p130 DIFFERENTLY
… effect of HR HPV 16 E7 and LR HPV 6 E7 on p130 intracellular localization and half-life was examined. Experiments were performed using human foreskin keratinocytes transduced with HPV 6 E7, HPV 16 E7 or parental vector. Nuclear/cytoplasmic fractionation and immunofluorescence showed that, in …
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Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast
… at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4<sup>+</sup>. We observed that 20% of the cells were undergoing division. Next we …
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Distribution of proteins involved in carbon catabolite repression in Aspergillus nidulans.
… The main aim of this study was to study the intracellular localization of proteins involved in CCR including CreA, CreB, CreC and CreD in A. nidulans in repressing and derepressing conditions. The major regulatory protein involved in CCR in A. nidulans is CreA. It is a DNA-binding repressor, …
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Analysis of the Nucleioprotein Complexes Essential for P1 Plasmid Partition
For all organisms, segregation and proper intracellular localization of DNA are essential processes in ensuring faithful inheritance of genetic material. In prokaryotes, several different mechanisms have developed for efficiently moving chromosomal DNA to proper cellular locations prior to cell …
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Mutations in Connexin43 and Connexin40 Associated with Human Atrial Fibrillation; Physiology and Mechanism
… The Cx43 frameshift mutant (Cx43-932delC) caused intracellular retention of Cx43, resulting in impaired electrical coupling between paired cells. All three Cx40 mutants (F30L, G311S and R113S) demonstrated normal trafficking and electrical coupling between paired N2A cells. Enhanced hemichannel …
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Regulation of the Intracellular Trafficking of Ajuba by Cell Density and Cell Cycle-Dependent Mechanisms
<p>The intracellular localization of Ajuba, a LIM domain-containing protein, holds essential value for various cellular activities. Previous studies from the laboratory have implicated Ajuba as a repressor of ATR in human cells. This study focused on unveiling the intricate mechanisms governing …
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Biochemical analyses of the Golgi and its associated membrane proteins in Saccharomyces cerevisiae
… These data were consistent with the proposed intracellular localization of these proteins to the yeast Golgi.
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Regulation of the Target of Rapamycin Signaling Pathway in Saccharomyces cerevisiae
… protein expression, and <em>in vivo</em> protein localization of fluorescence tagged proteins was employed to determine the function of an essential protein, Lst8, in TORC1 and TORC2 signaling and a previously uncharacterized complex, the Far3-7-8-9-10-11 complex (Far complex) in the budding …
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Expression and cellular localization of Copper transporter 2 (Ctr2) in Mus musculus
… mouse Ctr2, we studied the expression and localization of endogenous mouse Ctr2 in cell culture and in mouse models to understand its regulation and function in copper homeostasis. By immunoblot we observed a regulation of mCtr2 protein levels in a copper and Ctr1 dependent way. Our …
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Cellular and Developmental Functions of The Xenopus Arvcf-Catenin:Kazrin Complex
… dimerization/oligomerization. In considering the intracellular localization of the xARVCF-catenin:xKazrin complex, I did not resolve xKazrinA in a larger ternary complex with cadherin, nor did I detect its co-precipitation with core desmosomal components. Instead, screening revealed that xKazrinA …
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Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells
Salmonella enterica species are intracellular bacteria causative agents of gastroenteritis and typhoid fever in humans. Pregnancy poses an increased risk of severe Salmonellosis in many mammalian species contributing to miscarriage and/or maternal illness. Previous studies indicated that Salmonella …
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THE ROLE OF PHOSPHODIESTERASE 3B IN CAMP-MEDIATED REGULATION OF INSULIN SECRETION
… of PDE3B in biphasic insulin secretion and its intracellular localization, and (iii) to investigate the mechanisms for regulation of PDE3B activity in ?-cells. It was previously shown that PDE3B attenuates glucose-stimulated insulin secretion and glucagon-like peptide-1 (GLP-1) …
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Unraveling the Molecular Mechanisms of Munc18 and Munc13 in Mast Cell Exocytosis
… is the key cognate syntaxin whose level and intracellular localization are regulated by Munc18. In addition, our data demonstrate that Munc13-4 plays an essential role in mast cell degranulation; mutational studies revealed that point mutations in C2 domains of Munc13-4 dramatically alter …
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Design, Synthesis, and Characterization of a Novel Class of Mitochondrial Delivery Vectors: Mitochondria-penetrating Peptides
… and synthetic amino acids, and monitoring the intracellular localization by confocal fluorescence microscopy, the requisite thresholds for achieving mitochondrial accumulation with a cationic peptide were elucidated. These systematic studies led to the development of a novel class of cationic …
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Control of protein adsorption, cell morphology and intracellular signal transduction using self-assembled monolayers of alkylthiolates on gold
… surface interactions at the extracellular and intracellular level. The goal of this research was to provide molecular level information on the surface determinants necessary to produce predictable, controllable biological responses to implanted materials. Various players in "outside-in" …
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