{"id":{"repo_id":"u-pacific","oai_identifier":"oai:scholarlycommons.pacific.edu:uop_etds-1714"},"canonical_url":"https://search.dev.ndltd.org/etd/u-pacific/oai:scholarlycommons.pacific.edu:uop_etds-1714","repository":{"repo_id":"u-pacific","name":"University of the Pacific","base_url":"https://scholarlycommons.pacific.edu/do/oai/"},"display":{"title":"Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast","abstract":"In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1<sup>+</sup> was tagged at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4<sup>+</sup>. We observed that 20% of the cells were undergoing division. Next we observed the effects that deleting ben1<sup>+</sup> would have on the functions of Mto1p and Mto2p. We setup crosses between these strains and observed their localization. We did not observe any specific Mto2-GFP localization in ben1Δ. cells. This suggests that Ben1p plays a role in the proper localization of a γ-TuC associated protein.","abstract_html":"In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1&lt;sup&gt;+&lt;/sup&gt; was tagged at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4&lt;sup&gt;+&lt;/sup&gt;. We observed that 20% of the cells were undergoing division. Next we observed the effects that deleting ben1&lt;sup&gt;+&lt;/sup&gt; would have on the functions of Mto1p and Mto2p. We setup crosses between these strains and observed their localization. We did not observe any specific Mto2-GFP localization in ben1Δ. cells. This suggests that Ben1p plays a role in the proper localization of a γ-TuC associated protein.","abstract_has_math":false,"creators":["Escotto, Benjamin Alan"],"institution":null,"degree_name":"Master of Science (M.S.)","degree_level":"Thesis - Pacific Access Restricted","degree_discipline":"Biological Sciences","degree_department":null,"school":null,"contributors":["Srinivas Venkatram"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009-01-01T08:00:00Z","date_published":"2009-01-01T08:00:00Z","updated_at":"2026-07-24T05:36:46Z","subjects":["Microtubules","Microtubules Cloning","Fungal proteins","Mutagenesis","Biology","Life Sciences"],"languages":[],"rights":[],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"https://scholarlycommons.pacific.edu/uop_etds/715","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Srinivas Venkatram"]},{"key":"dc:creator","label":"Author","values":["Escotto, Benjamin Alan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2009-01-01T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - Pacific Access Restricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (M.S.)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microtubules","Microtubules Cloning","Fungal proteins","Mutagenesis","Biology","Life Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarlycommons.pacific.edu/uop_etds/715"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1<sup>+</sup> was tagged at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4<sup>+</sup>. We observed that 20% of the cells were undergoing division. Next we observed the effects that deleting ben1<sup>+</sup> would have on the functions of Mto1p and Mto2p. We setup crosses between these strains and observed their localization. We did not observe any specific Mto2-GFP localization in ben1Δ. cells. This suggests that Ben1p plays a role in the proper localization of a γ-TuC associated protein."]},{"key":"dc:source","label":"Dc Source","values":["61"]},{"key":"dc:title","label":"Title","values":["Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast"]}]}],"canonical_facts":{"dc:contributor":["Srinivas Venkatram"],"dc:creator":["Escotto, Benjamin Alan"],"dc:date.available":["2009-01-01T08:00:00Z"],"dc:description.abstract":["In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1<sup>+</sup> was tagged at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4<sup>+</sup>. We observed that 20% of the cells were undergoing division. Next we observed the effects that deleting ben1<sup>+</sup> would have on the functions of Mto1p and Mto2p. We setup crosses between these strains and observed their localization. We did not observe any specific Mto2-GFP localization in ben1Δ. cells. This suggests that Ben1p plays a role in the proper localization of a γ-TuC associated protein."],"dc:identifier":["https://scholarlycommons.pacific.edu/uop_etds/715"],"dc:rights":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:source":["61"],"dc:subject":["Microtubules","Microtubules Cloning","Fungal proteins","Mutagenesis","Biology","Life Sciences"],"dc:title":["Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast"],"thesis:degree_discipline":["Biological Sciences"],"thesis:degree_level":["Thesis - Pacific Access Restricted"],"thesis:degree_name":["Master of Science (M.S.)"]},"updated_at":"2026-07-24T05:36:46Z"}