Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1092 for “"Mutagenesis"”.
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Mapping the Genomic Context of Mutagenesis
… variants and other mutation types. However, mutagenesis is a multifaceted event that is affected by the genomic organisation of DNA and cellular processes such as transcription, replication, and DNA repair processes. Moreover, since many mutational processes also generate characteristic multi …
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Catalytic site mutagenesis of cytochrome P450 6B1v1
Cytochromes P-450 form a large superfamily of heme-containing monooxygenases that are found in species ranging from bacteria to insects, plants, and mammals. In insects, P-450s have several functional roles, including growth, development, feeding, resistance to pesticides, and tolerance to plant …
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Histone Deacetylase 1: Mutagenesis And Small Molecule Studies
<p>Histone deacetylase 1 (HDAC1) has been linked to cell growth and cell cycle regulation, which makes it a widely recognized target for anticancer drugs. The 14 Å channel of Class 1 HDAC isotypes has long being hypothesized to be the exit cavity for acetate following deacetylation. The amino acids …
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Quinolone mechanism of action: sensitivity, mutagenesis and tolerance
… of the SOS response, which allows for increased mutagenesis and the potential for increased antimicrobial resistance, thus making quinolones an interesting class of antibiotics to study. Although quinolones are one of the most effective classes of antibiotics, there are many conditions in which …
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Investigating the regulation of APOBEC mutagenesis in cancer
… thesis investigates the regulation of APOBEC mutagenesis in cancer. There is a focus on APOBEC3A and APOBEC3B as likely mediators of APOBEC signature mutations, and a focus on the hypothesis that APOBEC activity might be driven by the activity of LINE-1 retrotransposons. Firstly, the thesis …
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Somatic mutagenesis in humans with deficient DNA repair
… summarises experiments that investigate somatic mutagenesis in a selection of normal tissue types from individuals with germline heterozygous mutations in the DNA polymerase genes POLE and POLD1. Chapter 4 summarises the investigation of somatic mutagenesis in normal tissues from individuals with …
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Site-Directed Mutagenesis in Francisella Tularensis by Allelic
… tools have been developed that have enabled mutagenesis of F. tularensis type A and type B stains. However, site-specific mutations remain difficult to execute or these methods generate random mutations. In this study a novel method was developed to create site-directed mutations in a …
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Mutagenesis of nifE and nifN from Azotobacter vinelandii
The products of nifE and nifN from Azotobacter vinelandii, which are involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co) co) from nitrogenase, have been analyzed using a variety of mutagenic techniques. NifE was the object of several site-specific, amino acid substitutions that …
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Towards Novel Methods of Mutagenesis for Histophilus somni
… genes responsible for virulence, an efficient mutagenesis system is needed. Mutagenesis of H. somni using allelic exchange is difficult due to its tight restriction modification system. Mutagenesis by natural transformation in Haemophilus influenzae is well established and may be enhanced by …
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Mechanism of Mismatch Repair Induced Mutagenesis in Somatic Hypermutation
B cells produce a diverse array of antibody specificities that are of low affinity during the initial phase of a humoral immune response. However, somatic hypermutation of the rearranged V region in the immunoglobulin locus generates new antibody affinities, accompanied by the selection of B cells …
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Insertional mutagenesis in zebrafish using a pseudotyped retroviral vector
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Site-directed mutagenesis of the ncd microtubule motor protein
… melanogaster</i>. PCR-mediated site-directed mutagenesis was utilized to introduce specific mutations into pET/MC6, a construct containing the motor domain of ncd. Six mutations were generated, two at glutamic acid residue 656, two at proline residue 649, one at arginine residue 623, and one …
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Zebrafish Mutagenesis Study of Host Determinants of Mycobacterial Infection Outcome
This thesis describes a large-scale forward genetic screen in zebrafish for host determinants of mycobacterial infection outcome that I participated in. Zebrafish larvae from families randomly mutagenized with N-ethyl-N-nitrosourea were infected with Mycobacterium marinum and monitored over time. …
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Design and implementation of computational systems based on programmed mutagenesis
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1998.
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Spectroscopic analysis and synthesis of nucleoside analogs for lethal mutagenesis
… (KP1212), employs the mechanism of lethal mutagenesis to avoid the problem of drug resistance and use the virus's high mutation rate against it. KP1212 is incorporated into DNA by HIV reverse transcriptase (RT) but mispairs with adenine instead of guanine, increasing the rate of purine …
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