Northern Michigan University
CHARACTERIZATION OF THERANOSTIC PEPTIDES FOR GLIOBLASTOMA MULTIFORME
Abstract
dc:description.abstract<p>Glioblastoma multiforme (GBM) is a type of primary CNS tumor in which viable treatment options do not exist. Standard of care including tumor resection, chemotherapy, and radiation does little to extend the 5-year survival expectancy past 5.1%. Herein, two small-peptide molecules with inherent antitumor activity, blood-brain barrier permeability, and capability for tumor-specific drug deliverance and intraoperative visualization (termed <em>theranostic)</em> were of focus. Confocal microscopy was employed to characterize<em> in vitro </em>specificity of chlorotoxin, a 4 kDa scorpion venom peptide, and rBSG, the recombinant 25 kDa non-glycosylated extracellular domain of <em>e</em>xtracellular <em>m</em>atrix <em>m</em>etalloproteinase <em>in</em>ducer (EMMPRIN; Basigin) isoform 2, toward U87 GBM and MSU 1.1 human foreskin fibroblast cell line labelling. A novel cDNA construct coding a recombinant chlorotoxin (rCTX) variant for periplasmic prokaryotic expression and histidine-tag purification was created. However, prokaryotic expression and purification of histidine-tagged rCTX was not obtainable. Confocal data also supports variable labelling activity of commercially sourced CTX and rBSG <em>in vitro</em> for both U87 and MSU1.1 cell lines. These data support further investigation of small-peptide theranostics for GBM treatment.</p>
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- Thesis
- Discipline thesis:degree_discipline
- Biology
- Year dc:date.available
- 2018
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Mellesmoen, Aaron
- Contributors dc:contributor
-
- Robert Belton, Ph.D.
Subjects
dc:subject × 15Identifiers
dc:identifier.*- Repository record dc:identifier
- https://commons.nmu.edu/theses/556
- OAI identifier oai:identifier
- oai:commons.nmu.edu:theses-1570