Vps1's Implication on Late Endosome-to-Vacuole Traffic

<p>Previous studies have shown Vps1 to be necessary for both sorting of soluble vacuolar proteins from the Golgi and retention of Golgi membrane proteins. I have recently found that Vps1 is implicated in membrane trafficking from the plasma membrane through the endosomal system to the vacuole. Loss of Vps1 resulted in accumulation of FM4-64 puncta that colocalize with a late endosomal marker (GFP-Pep12), indicating a slower late endosome-to-vacuole traffic. In addition, Vps1 was found to genetically interact with Vps36 and Ypt7, which are involved in the late endosome-to-vacuole traffic. Protein's functional role is generally correlated with its localization, so I investigated the subcellular localization of Vps1-GFP in relation to endocytic sites and endosomal compartments. In a FM4-64 pulse-chase experiment, I found that the extent of colocalization of FM4-64 with Vps1-GFP drastically increased after 20 min chase, indicating Vps1-carrying vesicles are among the population of late endosomal compartments. Consistently, I observed that Vps1-GFP coincides with PI3P (endosomal marker), but not with the plasma membrane endocytic markers such as Abp1 and Ede1, suggesting that Vps1 is a component of endosomal compartments. Taken together, I postulate that Vps1 is required for an efficient traffic toward the vacuole from late endosomal compartments.</p>

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

2012

Author dc:creator

• Hayden, Jacob Robert

Contributors dc:contributor

• Kyoungtae Kim

• Vps1
• endosome
• vacuole
• trafficking
• FM4-64
• Biology

Statement dc:rights

• © Jacob Robert Hayden

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/1298

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-2299