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Showing 1 to 8 of 8 for “"Vps1"”.

  1. VPS1 on Dynamics of Endocytic Components and Endocytic Scission

    The yeast dynamin-related protein Vps1 shares high homology with dynamin in its N-terminal GTPase and C-terminal GED domains. To test the significance of two conserved domains in early endocytosis, an endocytic maker, Abp1, was fused with GFP in three GTPase mutants (vps1K42E, vps1S43N, and …

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  2. Vps1's Implication on Late Endosome-to-Vacuole Traffic

    <p>Previous studies have shown Vps1 to be necessary for both sorting of soluble vacuolar proteins from the Golgi and retention of Golgi membrane proteins. I have recently found that Vps1 is implicated in membrane trafficking from the plasma membrane through the endosomal system to the vacuole. Loss …

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  3. Novel Physical Interactions of Vps1 with Major Intracellular Traffic Regulators

    Vacuolar Protein Sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTPase that acts as a pivotal component in membrane trafficking. In light of observations that VPS1 displayed positive genetic interactions with four genes implicated in the recycling traffic (YPT6, GYP1, RCY1, and RIC1) …

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  4. Vps1's Role From the Early Endosome to the Golgi

    Vps1 is a dynamin-like protein that has been implicated in various cellular events, including protein trafficking from the late Golgi-to-endosome, peroxisome fission, vacuole fission and fusion, and retention of Golgi proteins. We recently observed in FM4-64 pulse-chase experiment with vps1 null …

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  5. Yeast Dynamin Functions with Clathrin at the Golgi

    Vps1 (vacuole protein sorting) is a GTPase protein and a yeast ortholog to the mammalian dynamin-like protein (Dlp1). Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. Classical dynamin family proteins work in …

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  6. Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi

    … late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal …

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  7. Functional Connection Between Yeast Dynamin and Retromer at the Endosome

    … the "Retromer” composed of 5 VPS proteins (Vps5, Vps17, Vps26, Vps29, and Vps35). Retromer acts as the coat proteins for vesicles emerging from late endosomes. Loss of Retromer function has been previously implicated in both Parkinson's and Alzheimer's disease. Vps1, a yeast dynamin-like protein, …

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  8. The Functional Synergy Between Yeast Dynamin and Key Membrane Recycling Factors

    … the late Golgi body. The dynamin-like GTPase Vps1p is required for vacuolar sorting and the late Golgi-retention of some proteins. It is known that the endocytic trafficking defect caused by the loss of VPS1 is essentially identical to cells that had lost RCY1. I hypothesized that VPS1 might …

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