Global ETD Search
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Showing 1 to 8 of 8 for “"FM4-64"”.
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Vps1's Implication on Late Endosome-to-Vacuole Traffic
… Loss of Vps1 resulted in accumulation of FM4-64 puncta that colocalize with a late endosomal marker (GFP-Pep12), indicating a slower late endosome-to-vacuole traffic. In addition, Vps1 was found to genetically interact with Vps36 and Ypt7, which are involved in the late endosome-to-vacuole …
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Vps1's Role From the Early Endosome to the Golgi
… of Golgi proteins. We recently observed in FM4-64 pulse-chase experiment with vps1 null cells that the levels of accumulated FM4-64-carrying transport vesicles increased significantly, suggesting the involvement of Vps1 on both the endocytic traffic to the vacuole, and the recycling traffic …
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Requirements of Slm Proteins For Proper Organization of Eisosome, Endocytic Trafficking, and Recycling
… of inactivated Slm proteins reveals that FM4-64 dye trafficking is inhibited at both permissive and non-permissive temperatures. Due to the fact that the severity in actin disruption is correlated with the severity of endocytic defects, I found that the inactivation of SLM genes led to …
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The Functional Synergy Between Yeast Dynamin and Key Membrane Recycling Factors
… defects of endocytic recycling to the membrane, FM4-64 dye, an endocytic vesicle marker, was used for visualization. We found that almost all double mutant cells accumulated dye in stages similar to vps1∆ cells. However, the rate at which FM4-64 was recycled out of the cell was found to be slower …
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Investigating the role of egg-3 by characterizing the egg-3(as40) phenotype in C. elegans
… egg-3(as40) was permeable to the lipophilic dye, FM4-64, at the restrictive temperature suggesting that there is a defect in the formation of the eggshell layer of the mutant. Calcofluor White staining also showed that the chitin layer fails to form in the mutants at 25°C. I have conducted two …
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Einfluss von Gibberellin auf den Gravitropismus der Wurzel durch Regulation der PIN-Stabilität
… erhöht werden. Da die Internalisierung von FM4-64, eines Endozytose-Indikators, durch veränderte GA-Gehalte nicht beeinflusst war, aber mehr PIN2 in BFA induzierten Endosomen akkumulierte, liegt der Schluss nahe, dass bei verstärkter GA-Bildung das PIN-Recycling vom PVC zurück zu den …
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Characterization of the first steryl ester transporter in biology, Yol075c, and its pleiotropic effects
… microscopy and the vacuolar specific dye FM4-64 was used to determine vacuole number and susceptibility to salt induced vacuole fission showing a slightly larger vacuole in the Yol075c cells which can be attributed to the observed but slight fission defect in the knockout. Both cell lines …
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Intracellular trafficking of Leishmania major peptidases
… with the endocytic / lysosomal marker FM4-64. Deletion of the LMP gene did not have any effect on promastigote growth in cell culture and only a small and transient slowing effect on the development of mouse footpad lesions after inoculation with LMP-deficient L. major. Lysosomal …