Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 121 for “"endosome"”.
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The kinetics of endosome processing
… marker. The pooling and/or segregation in the endosome was measured by determining co-localization with HRP. Colocalization of the two markers in the endosome is studied by using the ability of HRP to catalyse the crosslinking of membrane marker in endosomes with DAB (3,3'-diaminobenzidine), …
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Vps1's Implication on Late Endosome-to-Vacuole Traffic
… marker (GFP-Pep12), indicating a slower late endosome-to-vacuole traffic. In addition, Vps1 was found to genetically interact with Vps36 and Ypt7, which are involved in the late endosome-to-vacuole traffic. Protein's functional role is generally correlated with its localization, so I …
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Functional Connection Between Yeast Dynamin and Retromer at the Endosome
<p>Intracellular trafficking from the late endosome to Golgi in cells is termed retrograde transport, essential for recycling of important macromolecules including cell membrane receptors. Retrograde transport is regulated by a family of proteins known as the "Retromer” composed of 5 VPS proteins …
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Vps1's Role From the Early Endosome to the Golgi
… protein trafficking from the late Golgi-to-endosome, peroxisome fission, vacuole fission and fusion, and retention of Golgi proteins. We recently observed in FM4-64 pulse-chase experiment with vps1 null cells that the levels of accumulated FM4-64-carrying transport vesicles increased …
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Structural and Functional Characterization of the Endosome-associated Deubiquitinating Enzyme AMSH
<p>The endosomal sorting complexes required for transport (ESCRT) machinery is a ubiquitin-dependent molecular mechanism made of up of four individual complexes: ESCRT-0, -I, -II, III, that is necessary for regulating the degradation of cell surface receptors directed towards the lysosome. Not only …
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The Tissue Specific Role of Endosome Associated Protein SNX27 in Colonic Inflammation and Tumorigenesis
SNX27, member of the sorting nexin (SNX) family, carries a unique PDZ domain and mediates recycling of endocytosed transmembrane proteins. SNX27 is critical for neurodevelopment and whole body knockout in mice has been found to be embryonically lethal. Although SNX27 is variably expressed in …
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Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi
… in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's …
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Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes
… in the endocytic pathway, in particular, early endosome processing and delivery to the lysosomes. Two models for endosome processing have previously been proposed in the literature, namely the maturation and vesicular traffic models. The general consensus has been an early phase of intermingling …
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Regulation of Traffic into and out of the Yeast Endosome by the VPS9P Cue Domain and the VPS5P Domain
… This receptor then facilitates transport to the endosome where the biosynthetic and endocytic pathways coincide. The receptor is recycled back to the Golgi while the vacuolar hydrolases and endocytic material are conveyed to their ultimate destination. I have been interested in two aspects of …
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The synthesis, characterization, and use of a protein-cysteine proteinase inhibitor complex for the study of endosome/lysosome fusion
The cysteine proteinases cathepsins B, L, and S are lysosomal enzymes responsible for the degradation of endocytosed proteins. Their presence in human cell monocytic lines THP1 and U937 was detected by the use of the membrane-permeable, irreversible, active-site directed inhibitor Fmoc-(¹²⁵I)Tyr- …
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The interconnected roles of spastin and protrudin in endosomal sorting
… is associated with endoplasmic reticulum (ER)-endosome membrane contact sites and several ER proteins are required for efficient endosomal tubule fission. Our lab has previously identified two hereditary spastic paraplegia (HSP)- associated proteins, spastin (SPG4) and atlastin-1 (SPG3A), as …
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Investigations into the Nature of the Endosomal System in Plasmodium falciparum
… well-conserved protein components of the late endosome, the retromer cargo-selective complex and Rab7, were found on a previously un-described inherited structure adjacent to the parasite Golgi apparatus and in close opposition to nascent rhoptries (specialized secretory organelles required for …
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Identification and Characterization of Effectors/Binding Molecules for the Small GTPase Rab15
… receptor internalization into early endosomes. Here internalized receptors are\r\nsorted for degradation in lysosomes, direct recycling back to the cell surface or indirect\r\nrecycling via a second recycling compartment called the pericentriolar recycling\r\nendosome. Rab GTPases …
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Characterization of Class D VPS Proteins
… traffic between the late Golgi and late endosome. Although most of these proteins have homologues of known function in other systems, two exceptions are the Vps3p and Vps8p proteins. Analysis of Vps3p reveals that it is associated with a highdensity structure, possibly a coated vesicle or …
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Endocytic Trafficking of The Amyloid Precursor Protein In Rat Cortical Neurons
… and their secretion remain unclear. Using endosomes isolated from cultured primary neurons, we determined that the trafficking of APP from the endosomal membrane into internal vesicles of late endosome/multivesicular bodies (MVB) is dependent on Endosomal Sorting Complexes Required for …
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In vitro reconstitution of Central Spindle Motility
… the asymmetric segregation of signalling endosomes containing fate determinants in only one daughter cell. A modest asymmetry in the density of microtubules within the antiparallel MT overlap of central spindle, with more MTs on the anterior side of the spindle, generates one order of …
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Synthesis of pH-responsive core-shell nanoparticles of different sizes and with different shell compositions
The endosome-disrupting and pH-responsive poly(2-diethylamino ethyl methacrylate)-core/poly(2- aminoethyl methacrylate)-shell nanoparticles could potentially increase the efficacy of transcutaneous administered vaccines and facilitate the cytosolic delivery of a wide variety of therapeutic …
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Structure/Function Studies on Vps9 domain-containing Ankyrin Repeat protein (VARP)
… utilises different SNX proteins to mediate both Endosome to Golgi traffic, and Endosome to Plasma membrane traffic. The interaction of Varp with Vps29 localises Varp to endosomal membranes. Varp has two ‘zinc knuckle’ sequences (residues 369- 460 and 692-746) that have been shown to bind zinc and …
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The Functional Synergy Between Yeast Dynamin and Key Membrane Recycling Factors
… materials from the trans-Golgi network or endosome back to the cell membrane, as opposed to being targeted to the vacuole for degradation. Ypt31p and Ypt32p GTPases are known to regulate the function of F-box protein Rcy1p, which mediates recycling of the plasma membrane v-SNARE Snc1p. …
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A novel pathway for VLDL assembly in the mouse liver
… wahrscheinlich dem TGN oder dem "recycling endosome", transportiert, um lipidiert und als VLDL, in geringerem Umfang auch als IDL, sezerniert zu werden. Diese Daten beweisen, dass als LDL endozytiertes apoB100 zur Synthese von VLDL wiederverwendet werden kann. Eine solche Wiederverwendung …
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