Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 135 for “"vacuole"”.
-
Vps1's Implication on Late Endosome-to-Vacuole Traffic
… membrane through the endosomal system to the vacuole. Loss of Vps1 resulted in accumulation of FM4-64 puncta that colocalize with a late endosomal marker (GFP-Pep12), indicating a slower late endosome-to-vacuole traffic. In addition, Vps1 was found to genetically interact with Vps36 and Ypt7, …
-
The feeding apparatus and the food vacuole in paramecium multimicronucleatum
Thesis (M.S.)--Michigan State College of Agriculture and Applied Science. Dept. of Zoology, 1951
-
Recruitment and function of ORP1L on the Coxiella burnetii parasitophorous vacuole
… niche, a large, lysosome-like parasitophorous vacuole (PV), is essential for bacterial survival and replication. There is growing evidence that host cell cholesterol trafficking plays a critical role in PV development and maintenance, prompting an examination of the role of cholesterol-binding …
-
Polyphosphoinositide-derived signals in the regulation of vacuole membrane fusion and fission
… exchange membrane and protein cargoes. The yeast vacuole is a highly dynamic organelle that changes shape in response to both hypo and hyper-osmotic stresses and so has been used to study both membrane fusion and fission. Fusion is relatively well characterised but fission is not well understood. …
-
The regulation of yeast homotypic vacuole fusion by phosphatidic acid and diacylglycerol
… mix. In Saccharomyces cerevisiae, homotypic vacuole fusion occurs through a series of well-defined phases. SNAREs are activated by their co-chaperones Sec18 (mammalian NSF) and Sec17 (mammalian α-SNAP) in an ATP dependent priming stage. Tethering first brings two fusing compartments into …
-
Mutational analysis of the Vam3 transmembrane domain and its effects on vacuole fusion in Saccharomyces cerevisiae
Vam3 is a SNARE protein essential in yeast vacuole homotypic fusion. The specific role of the Vam3 trans-membrane domain (TMD) during vacuole fusion remains unclear. Previous work had shown that replacing Vam3 TMD with a prenyl lipid anchor (CCIIM) results in attenuated fusion and accumulation of …
-
Lipid composition and membrane physical parameters regulate priming and SNARE function in yeast vacuole membrane fusion
… report that inducing negative curvature on the vacuole membrane lowers the force threshold required for fusion, thus allowing non-canonical SNARE complexes to support fusion. On the other hand, increasing the positive curvature of the membrane increases the force threshold such that even …
-
Biochemical Characterization of Two Aminopeptidases Involved in Hemoglobin Catabolism in the Food Vacuole of Plasmodium falciparum
… to its acidic organelle, known as the food vacuole, where a series of proteases degrade a majority of the host hemoglobin. Two metalloaminopeptidases, PfAPP and PfA-M1, have been previously localized to the food vacuole (in addition to distinct secondary locations for each), implicating them …
-
The regulation of yeast homotypic vacuole fusion and membrane trafficking events by sphingolipids, ergosterol, and lipid rafts
… pro-fusion proteins and regulatory lipids at the vacuole, wherein both interacting partners are required for proper localization to specific membrane domains. Regulatory lipids and select fusion proteins enrich in the vertex ring domain of vacuoles that are in the process of tethering and docking …
-
The role of the Dgk1 and Pah1 in lipid interconversion on saccharomyces cerevisiae vacuole physiology, homeostasis, protein sorting and fusion
… are needed for the homotypic fusion of the yeast vacuole, including a number of proteins and regulatory lipids. The deletion of the phosphatidic acid (PA) phosphatase gene Pah1, which converts PA in diacylglycerol kinase (DAG), was previously shown to produce a fragmented vacuole phenotype, …
-
A metallomic, proteomic and lipidomic investigation of the malaria parasites digestive vacuole and insights into the mediators of haemozoin formation
… in their release from purified digestive vacuoles (DVs) following multiple freeze-thaw cycles. Isolated crystals were extensively washed with aqueous sodium acetate buffer (0.5 M, pH 5.2), detergent(4% SDS) and organic solvents (acetone/methanol) prior to base dissolution (0.1 M NaOH). …
-
Anaplasma phagocytophilum remodels its host cell-derived vacuole into a protective niche by redecorating the vacuolar membrane with select Rab GTPases and bacterial proteins
… pathogen replicates within a host cell-derived vacuole that fails to mature along the endocytic pathway, does not acidify, and does not fuse with lysosomes. Selective fusogenicity is prototypical of many vacuole-adapted pathogens and has been attributed, at least in part, to pathogen …
-
Osmoregulation in Trypanosoma Cruzi: The Role of the Acidocalcisome
… microscopic observations revealed a contractile vacuole coupled to acidocalcisomes in all three life cycle stages of the parasite. An aquaporin homologue localized to the acidocalcisomes and contractile vacuole. In epimastigotes expressing a GFP-aquaporin fusion protein, acidocalcisomes …
-
Development and application of a high throughput cell based assay to identify apoptosis inducing proteins, and functional characterization of the candidate Vacuole Membrane Protein 1 (Vmp1)
… activators of apoptosis. One of the candidates, Vacuole Membrane Protein 1 (Vmp1), which forms vacuoles in cells and subsequently induces apoptosis when overexpressed, has been functionally characterized in detail. It has been reported that VMP1 mRNA is differentially expressed in cancer, acute …
-
The regulation of yeast homotypic membrane fusion by class C ABC transporters
… fusion. In Saccharomyces cerevisiae, homotypic vacuole fusion occurs in experimentally defined phases. Fusion priming does not involve contact between vacuoles but includes the disassembly of complexes of SNAREs on the same membrane (cis) by Sec18p (NSF) and its cochaperone Sec17p (a-SNAP). …
-
Investigation of prey ingestion and digestion in the ciliate predator Tetrahymena pyriformis and the impact on growth
… of four prey states on ingestion, digestive vacuole (DV) formation, digestion and growth in the ciliate Tetrahymena pyriformis. The prey included several strains of live RFP-expressing heterotrophic bacteria (Live), heat-killed-DTAF-stained heterotrophic bacteria (Dead), live autotrophic …
-
Chloroquine accumulation in Plasmodium falciparum isolated digestive vacuoles
… intact Plasmodium falciparum isolated digestive vacuoles, the site of chloroquine accumulation and action. Marker enzymes and gel electrophoresis were used to demonstrate purity, and electron microscopy to verify integrity of isolated vacuoles. Using this method, vacuoles were isolated in a yield …
-
Isolation and characterization of Saccharomyces cerevisiae mutants for vacuolar import and autophagocytosis
… a cytosolic procursor that is transported to the vacuole via the cytoplasm to vacuole trnasport (Cvt) pathway. The cytosolic protein is enclosed in a double-membrane vesicle, which is transported to and fuses with the vacuole releasing a single-membrane autophagic body into the vacuolar lumen. …
-
Regulation of GAP1 permease sorting in Saccharomyces cerevisiae
… nitrogen source, Gap p is sorted to the vacuole and degraded. To better understand the signals governing Gaplp sorting, we isolated mutants with defects in Gaplp sorting during growth on a non-preferred nitrogen source. We found that genes that regulate the glutamate and glutamine …
-
Investigations into the Nature of the Endosomal System in Plasmodium falciparum
… specialized acidic organelle, known as the food vacuole, where the parasite catabolizes the majority of its host cell hemoglobin. We analyzed the effects of a PfFCP dominant negative mutant and found that it altered food vacuole morphology and trafficking. A previous report localized the early …
Page 1 of 7