Effect of Intranasal Delivery of Carbon Dioxide on Trigeminal Ganglion Neurons: Inhibition of Neuron-Glia Gap Junction Communication and Neuropeptide Secretion

<p>The data from recent clinical trails have reported that intranasal administration of non-inhaled 100% medical grade CO2 is an effective and safe treatment for migraine and allergic rhinosinusitis, but the exact mechanism of action of CO2 in treating these diseases is not well understood. Activation of trigeminal nerves, release of CGRP, and neuron-glia interactions through gap junctions in trigeminal ganglion are implicated in the pathology of migraine and allergic rhinosinusitis. The goal of this study was to determine whether administration of 100% CO2 to the nasal mucosa of rats inhibits trigeminal ganglion neuron-glia signaling and neuronal cell secretion. Increased signaling via gap junctions between trigeminal neurons and satellite glial cells was seen 2 hrs after capsaicin injection into the whisker pad. This gap junction communication was greatly reduced in animals treated with CO2 prior to capsaicin stimulation. CO2 treatment also blocked the stimulatory effect of capsaicin on Cx 26, S100B and CGRP expression. In addition, CO2 caused a decrease in levels of SNAP-25, a protein involved in controlling CGRP release from trigeminal nerves. Results from this in vivo study provide the first evidence of a unique regulatory mechanism by which CO2 inhibits sensory nerve activation, neuron-glial cell signaling, and subsequent neuropeptide release.</p>

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

2008

Author dc:creator

• Kankipati, Stanka Madhu Kumar

Contributors dc:contributor

• Paul Durham

• neuron-glia interactions
• gap junctions
• Cx 26
• CGRP
• SNAP-25
• S100B
• trigeminal
• Biology

Statement dc:rights

• © Stanka Madhu Kumar Kankipati

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/1256

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-2257