Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"SNAP-25"”.
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Palmitoylation and Oxidation of the Cysteine Rich Region of SNAP-25 and their Effects on Protein Interactions
… leads to exocytosis. The secondary structure of SNAP-25 is unique among SNARE proteins in that it has two alpha helical SNARE motifs and a cysteine rich (C85, C88, C90, C92) membrane interacting region but notransmembrane domain. The cysteines may be modified by palmitoylation or oxidation but …
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Fluoreszenzmikroskopische Studien an Plasmamembranen zur Untersuchung der molekularen Mechanismen der neuronalen Exocytose
… den plasmamembranständigen SNAREs Syntaxin1A und SNAP-25 und dem vesikelassoziierten SNARE Synaptobrevin. Dadurch werden die gegenüberliegenden Membranen fusioniert. Neben den SNAREs ist auch Munc18-1, das zur Sec1/Munc18-Proteinfamilie gehört, ein essentielles Protein der neuronalen Exocytose. …
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Fluoreszenzmikroskopische Studien an Plasmamembranen zur Untersuchung der molekularen Mechanismen der neuronalen Exocytose
… den plasmamembranständigen SNAREs Syntaxin1A und SNAP-25 und dem vesikelassoziierten SNARE Synaptobrevin. Dadurch werden die gegenüberliegenden Membranen fusioniert. Neben den SNAREs ist auch Munc18-1, das zur Sec1/Munc18-Proteinfamilie gehört, ein essentielles Protein der neuronalen Exocytose. …
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Dynamic changes in the localization of synapse associated proteins during development and differentiation of the mammalian retina
… terminal membrane associated proteins SNAP-25 and Syntaxin, to characterize their spatio-temporal distribution during retinal differentiation and in the mature retina;The vertebrate retina has a laminar organization consisting of three cellular layers separated by two synaptic layers, …
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Studien zu den biochemischen und strukturellen Eigenschaften von Snapin und zu seiner Rolle in der neuronalen Exozytose
Snapin wurde ursprünglich von Ilardi et., al 1999 als hirnspezifisches Protein beschrieben, das mit SNAP-25, einem zentralen Protein der neuronalen Exozytse, interagiert. Sowohl biochemische Daten über die Bindung an SNAP-25 und Einfluss von Snapin auf die Interaktion zwischen dem SNARE-Komplex und …
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Insights into the Functions of Munc18-1 in Neurotransmitter Release
… Munc18-1 and the SNARE proteins syntaxin-1, SNAP-25 and synaptobrevin. The SNAREs form tight SNARE complexes that bring the vesicle membrane and plasma membrane into close proximity and provide forces to induce membrane fusion. Munc18-1 is essential because deletion of Munc18-1 in mice leads …
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Biophysikalische und thermodynamische Charakterisierung der neuronalen SNARE-Komplexbildung
… Dabei bilden die plasmamembranständigen Proteine SNAP-25 und Syntaxin einen Akzeptorkomplex für das in der Vesikelmembran sitzende Synaptobrevin aus. Man geht davon aus, dass sich aufgrund dieser Komplexbildung die Membranen annähern und so letztendlich deren Fusion induziert wird. Der genaue …
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SNAREs in evoked and spontaneous neurotransmission
Der SNARE-Komplex, bestehend aus Synaptobrevin-2, SNAP-25 und Syntaxin-1 ist unabdingbar für evozierte synaptische Übertragung, allerdings ist der Einfluss der möglichen Intermediärstadien des SNARE-Komplexes in den verschiedenen Phasen der synaptischen Übertragung unbekannt. Mittels Mutagenese von …
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Mechanical Properties of Individual Molecules : An Interface between the Structure and the Function of the Molecules
… of three proteins: syntaxin, synaptobrevin and SNAP-25. We measured and analyzed the mechanical properties of the interactions between four combinations between the building blocks of the SNARE-complex. Altogether, four different protein combinations were measured. The molecular binding assay …
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The SEC9 gene is essential for exocytosis in yeast and encodes a dispensable amino terminal domain
… a statistically significant similarity to SNAP-25, a synaptosomal associated protein. The sec9-4 gene bears a single basepair substitution, in the C-terminal domain, that changes a G residue to an A residue. A corresponding amino acid substitution occurs and changes residue 458 from glycine …
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The Fourier convolution-deconvolution method: its limitation and application
… distance within the C-terminus region of the SNAP-25 protein.</p>
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… the SNARE proteins synaptobrevin, syntaxin and SNAP-25, as well as Munc18. Besides these universal components, many other neuronal specific proteins are also involved in regulating Ca2+-triggered release. Interestingly, most of these regulatory proteins contain C2 domains, versatile protein …
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Unraveling the Role of SNARE Interactions in Neurotransmitter Release
… and the plasma membrane proteins syntaxin 1 and SNAP-25, which are collectively known as SNAREs and form a tight complex (the core complex). The assembly of the core complex may mediate membrane fusion. Complexin is a highly conserved cytoplasmic protein that binds tightly to the SNARE complex. …
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Excessive iron accumulation mediated oxidative stress and β-cell dysfunction via perturbations of insulin secretion in MIN6 cells
… 48 h, insulin secretion & content, expression of SNAP-25, mitochondrial oxygen consumption rate (OCR), mitochondrial membrane potential (Δψm), and protein carbonylation were estimated. In addition, antioxidant activity (cellular/non-cellular), cytotoxicity, and cellular ferritin were assessed …
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Effect of Intranasal Delivery of Carbon Dioxide on Trigeminal Ganglion Neurons: Inhibition of Neuron-Glia Gap Junction Communication and Neuropeptide Secretion
… In addition, CO2 caused a decrease in levels of SNAP-25, a protein involved in controlling CGRP release from trigeminal nerves. Results from this in vivo study provide the first evidence of a unique regulatory mechanism by which CO2 inhibits sensory nerve activation, neuron-glial cell signaling, …
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Effect of hypoxia on spontaneous neural activity in the cortex of neonate mouse pups
… to bregma) under normal air and hypoxic air in SNAP-25-2A-GCaMP-6s transgenic mice at postnatal day 7. Results of this study demonstrated a statistically significant reduction in frequency and increase in amplitude of neuronal activity in the entire cortex under hypoxic air when compared to …
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Effect of hypoxia on spontaneous neural activity in the cortex of neonate mouse pups
… to bregma) under normal air and hypoxic air in SNAP-25-2A-GCaMP-6s transgenic mice at postnatal day 7. Results of this study demonstrated a statistically significant reduction in frequency and increase in amplitude of neuronal activity in the entire cortex under hypoxic air when compared to …
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Alcohol alters the expression of Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptors (SNAREs) and spontaneous γ-Aminobutyric Acid (GABA) release...
… 1 and synaptosomal associated protein of 25 kDa, which is also known as SNAP-25), and their interactions within the SNARE complex trigger vesicle fusion and neurotransmitter release. We found that ethanol treatment of mouse cortical neurons increased the mRNA and protein expression levels …
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Characterization of the Fast Axonally Transported Proteins in the Rat Optic Pathway
… labeled protein in the rat optic pathway (SNAP-25) was found to be associated with specific subsets of FT and slow transported (SCb) proteins. In summary, the FT proteins in the rat optic pathway displayed heterogenous transport initiation, kinetics, compartmentation, and intermolecular …
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The establishment and characterization of an improved cell-free assay for exocytosis in neuroendocrine PC12 cells
… durch die SNARE-Proteine Synaptobrevin/VAMP, SNAP-25 and Syntaxin1 vermittelt. Während der Fusion bilden diese SNARE-Proteine einen stabilen Komplex, der anschließend in einer konzertierten Aktion von α-SNAP und der AAA-ATPase NSF dissoziiert wird. In dieser Arbeit wurde ein zellfreier …
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