ENGINEERING LIPID AND HYBRID NANOVECTORS FOR TARGETED GENE DELIVERY TO MACROPHAGE SUBPOPULATIONS

In this work, a structural taxonomy of these systems is proposed with the aim of clarifying the possible architectures that can arise when genetic materials are complexed with a polycation or a lipid, followed by the addition of a lipid or a polymer to ameliorate the cellular toxicity, modulate cellular uptake and/or improve the transfection efficiency of the resulting nanovectors (Chapter 1). The experimental part of this thesis is organized presenting first the technological data of the prepared lipopolyplexes (Chapter 2), PLA-PEG/lipid nanoparticles (Chapter 3) and LNP (Chapter 4) and then their in vitro performances on polarized macrophages isolated from human blood (Chapter 5).

Grantor dc:publisher

Università degli Studi di Milano

Year dc:date

2026

Author dc:creator

• POZZA, CAMILLA

Contributors dc:contributor

• tutor: F. Cilurzo; co-tutor: L. Rizzello
• S. Pellegrino
• C. Pozza
• CILURZO, FRANCESCO

• macrophage
• targeted gene delivery
• non-viral vector
• nucleic acid delivery
• mRNA delivery
• Settore CHEM-07/A - Chimica farmaceutica

Statement dc:rights

• info:eu-repo/semantics/embargoedAccess
• license:Creative commons
• license uri:http://creativecommons.org/licenses/by-sa/4.0/

Language dc:language

eng

Handle dc:identifier

https://hdl.handle.net/2434/1250215

OAI identifier oai:identifier

oai:air.unimi.it:2434/1250215