{"id":{"repo_id":"milano","oai_identifier":"oai:air.unimi.it:2434/1250215"},"canonical_url":"https://search.dev.ndltd.org/etd/milano/oai:air.unimi.it:2434/1250215","repository":{"repo_id":"milano","name":"Università degli Studi di Milano","base_url":"https://air.unimi.it/oai/request"},"display":{"title":"ENGINEERING LIPID AND HYBRID NANOVECTORS FOR TARGETED GENE DELIVERY TO MACROPHAGE SUBPOPULATIONS","abstract":"In this work, a structural taxonomy of these systems is proposed with the aim of clarifying the possible architectures that can arise when genetic materials are complexed with a polycation or a lipid, followed by the addition of a lipid or a polymer to ameliorate the cellular toxicity, modulate cellular uptake and/or improve the transfection efficiency of the resulting nanovectors (Chapter 1). The experimental part of this thesis is organized presenting first the technological data of the prepared lipopolyplexes (Chapter 2), PLA-PEG/lipid nanoparticles (Chapter 3) and LNP (Chapter 4) and then their in vitro performances on polarized macrophages isolated from human blood (Chapter 5).","abstract_html":"In this work, a structural taxonomy of these systems is proposed with the aim of clarifying the possible architectures that can arise when genetic materials are complexed with a polycation or a lipid, followed by the addition of a lipid or a polymer to ameliorate the cellular toxicity, modulate cellular uptake and/or improve the transfection efficiency of the resulting nanovectors (Chapter 1). The experimental part of this thesis is organized presenting first the technological data of the prepared lipopolyplexes (Chapter 2), PLA-PEG/lipid nanoparticles (Chapter 3) and LNP (Chapter 4) and then their in vitro performances on polarized macrophages isolated from human blood (Chapter 5).","abstract_has_math":false,"creators":["POZZA, CAMILLA"],"institution":"Università degli Studi di Milano","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["tutor: F. Cilurzo; co-tutor: L. Rizzello","S. Pellegrino","C. 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The experimental part of this thesis is organized presenting first the technological data of the prepared lipopolyplexes (Chapter 2), PLA-PEG/lipid nanoparticles (Chapter 3) and LNP (Chapter 4) and then their in vitro performances on polarized macrophages isolated from human blood (Chapter 5)."],"dc:identifier":["https://hdl.handle.net/2434/1250215"],"dc:language":["eng"],"dc:publisher":["Università degli Studi di Milano"],"dc:relation":["numberofpages:157"],"dc:rights":["info:eu-repo/semantics/embargoedAccess","license:Creative commons","license uri:http://creativecommons.org/licenses/by-sa/4.0/"],"dc:subject":["macrophage","targeted gene delivery","non-viral vector","nucleic acid delivery","mRNA delivery","Settore CHEM-07/A - Chimica farmaceutica"],"dc:title":["ENGINEERING LIPID AND HYBRID NANOVECTORS FOR TARGETED GENE DELIVERY TO MACROPHAGE SUBPOPULATIONS"],"dc:type":["info:eu-repo/semantics/doctoralThesis"]},"updated_at":"2026-07-27T20:19:12Z"}