Expression of E-cadherin and HGF in Hypopharyngeal Cancer

BACKGROUND AND OBJECTIVES: Head and neck squamous cell carcinoma (HNSCC) results from a multistep carcinogenesis process in which increasing degrees of mucosal changes and cellular atypia occur over large areas of epithelium. Hypopharyngeal squamous cell carcinoma(SCC) is well known as a aggressive cancer which has relatively poor prognosis. E-cadherin is a large family of Calcium dependent intercelluar Adhesion Molecule(CAM) whose functions are essential for the induction and maintenance of intercellular cohesion and hepatocyte growth factor(HGF), a potent stimulator of hepatocyte growth, stimulates motility, invasiveness, proliferation, and morphogenesis of epithelium, and also may be involved in tumor invasion. So we examined the role of the E-cadherin/HGF on invasion and metastasis of hypopharyngeal squamous cell carcinoma(SCC). MATERIALS AND METHOD: We performed immunohistochemical stains on 40 tissues of hypopharyngeal cancer patients who were admitted to Ajou University Hospital & Severance Hospital between Mar. 1994 and Dec. 2003 with E-cadherin antibody. We classified the stains according to the intensity and location of E-cadherin expression, and analysis the correlation between the intensity or location of E-cadherin expression and the many clinicopathologic factors. To find the effect of HGF for the E-cadherin expression in the progression of hypopharyngeal cancer, RT-PCR and Western blot in hypopharyngeal cell line(FaDu) were performed according to the concentration of HGF(0, 10, 30 ng/ml). Hypopharyngeal cancer cell line RESULTS: All tissues were positive for the immunohistochemical staining of E-cadherin. According to the intensity +, ++ and +++ group were 25%, 55% and 20% respectively and according to the location membranous and non-membranous group were 50% respectively. Although the intensity of E-cadherin was not correlate with any clinical factor, the location of E-cadherin was siginificantly correlated with lymph node metastasis(p<0.05). Analyze for RT-PCR and Western Blot of E-cadherin expression in FaDu cells revealed the decreased expression of E-cadherin in proportion to the concentration of HGF. CONCLUSION: These results suggest that non-membranous expression of E-cadherin in hypopharyngeal cancer was significantly correlated with lymph node metastasis and that HGF may plays an important role in progression of hypopharyngeal cancer through significantly reducing the expression of E-cadherin.

Author dc:creator

• 이, 장우

Contributors dc:contributor

• 김, 철호
• 대학원 의학과
• 200324513

• E-cadherin
• 간세포성장인자
• 하인두암
• 침습
• 전이

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001297
000000001297

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1577