{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/1577"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/1577","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"Expression of E-cadherin and HGF in Hypopharyngeal Cancer","abstract":"BACKGROUND AND OBJECTIVES: Head and neck squamous cell carcinoma (HNSCC) results from a multistep carcinogenesis process in which increasing degrees of mucosal changes and cellular atypia occur over large areas of epithelium. Hypopharyngeal squamous cell carcinoma(SCC) is well known as a aggressive cancer which has relatively poor prognosis. E-cadherin is a large family of Calcium dependent intercelluar Adhesion Molecule(CAM) whose functions are essential for the induction and maintenance of intercellular cohesion and hepatocyte growth factor(HGF), a potent stimulator of hepatocyte growth, stimulates motility, invasiveness, proliferation, and morphogenesis of epithelium, and also may be involved in tumor invasion. So we examined the role of the E-cadherin/HGF on invasion and metastasis of hypopharyngeal squamous cell carcinoma(SCC). MATERIALS AND METHOD: We performed immunohistochemical stains on 40 tissues of hypopharyngeal cancer patients who were admitted to Ajou University Hospital & Severance Hospital between Mar. 1994 and Dec. 2003 with E-cadherin antibody. We classified the stains according to the intensity and location of E-cadherin expression, and analysis the correlation between the intensity or location of E-cadherin expression and the many clinicopathologic factors. To find the effect of HGF for the E-cadherin expression in the progression of hypopharyngeal cancer, RT-PCR and Western blot in hypopharyngeal cell line(FaDu) were performed according to the concentration of HGF(0, 10, 30 ng/ml). Hypopharyngeal cancer cell line RESULTS: All tissues were positive for the immunohistochemical staining of E-cadherin. According to the intensity +, ++ and +++ group were 25%, 55% and 20% respectively and according to the location membranous and non-membranous group were 50% respectively. Although the intensity of E-cadherin was not correlate with any clinical factor, the location of E-cadherin was siginificantly correlated with lymph node metastasis(p<0.05). Analyze for RT-PCR and Western Blot of E-cadherin expression in FaDu cells revealed the decreased expression of E-cadherin in proportion to the concentration of HGF. CONCLUSION: These results suggest that non-membranous expression of E-cadherin in hypopharyngeal cancer was significantly correlated with lymph node metastasis and that HGF may plays an important role in progression of hypopharyngeal cancer through significantly reducing the expression of E-cadherin.","abstract_html":"BACKGROUND AND OBJECTIVES: Head and neck squamous cell carcinoma (HNSCC) results from a multistep carcinogenesis process in which increasing degrees of mucosal changes and cellular atypia occur over large areas of epithelium. Hypopharyngeal squamous cell carcinoma(SCC) is well known as a aggressive cancer which has relatively poor prognosis. E-cadherin is a large family of Calcium dependent intercelluar Adhesion Molecule(CAM) whose functions are essential for the induction and maintenance of intercellular cohesion and hepatocyte growth factor(HGF), a potent stimulator of hepatocyte growth, stimulates motility, invasiveness, proliferation, and morphogenesis of epithelium, and also may be involved in tumor invasion. So we examined the role of the E-cadherin/HGF on invasion and metastasis of hypopharyngeal squamous cell carcinoma(SCC). MATERIALS AND METHOD: We performed immunohistochemical stains on 40 tissues of hypopharyngeal cancer patients who were admitted to Ajou University Hospital & Severance Hospital between Mar. 1994 and Dec. 2003 with E-cadherin antibody. We classified the stains according to the intensity and location of E-cadherin expression, and analysis the correlation between the intensity or location of E-cadherin expression and the many clinicopathologic factors. To find the effect of HGF for the E-cadherin expression in the progression of hypopharyngeal cancer, RT-PCR and Western blot in hypopharyngeal cell line(FaDu) were performed according to the concentration of HGF(0, 10, 30 ng/ml). Hypopharyngeal cancer cell line RESULTS: All tissues were positive for the immunohistochemical staining of E-cadherin. According to the intensity +, ++ and +++ group were 25%, 55% and 20% respectively and according to the location membranous and non-membranous group were 50% respectively. Although the intensity of E-cadherin was not correlate with any clinical factor, the location of E-cadherin was siginificantly correlated with lymph node metastasis(p<0.05). Analyze for RT-PCR and Western Blot of E-cadherin expression in FaDu cells revealed the decreased expression of E-cadherin in proportion to the concentration of HGF. CONCLUSION: These results suggest that non-membranous expression of E-cadherin in hypopharyngeal cancer was significantly correlated with lymph node metastasis and that HGF may plays an important role in progression of hypopharyngeal cancer through significantly reducing the expression of E-cadherin.","abstract_has_math":false,"creators":["이, 장우"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["김, 철호","대학원 의학과","200324513"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-03-08","date_published":"2011-03-08","updated_at":"2026-07-24T00:51:22Z","subjects":["E-cadherin","간세포성장인자","하인두암","침습","전이"],"languages":["ko"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001297","000000001297"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001297","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001297","code":true},{"text":"000000001297","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/1577","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["김, 철호","대학원 의학과","200324513","이, 장우"]},{"key":"dc:creator","label":"Author","values":["이, 장우"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-03-08","2006"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["E-cadherin","간세포성장인자","하인두암","침습","전이"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ko"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/1577","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001297","000000001297"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["BACKGROUND AND OBJECTIVES: Head and neck squamous cell carcinoma (HNSCC) results from a multistep carcinogenesis process in which increasing degrees of mucosal changes and cellular atypia occur over large areas of epithelium. Hypopharyngeal squamous cell carcinoma(SCC) is well known as a aggressive cancer which has relatively poor prognosis. E-cadherin is a large family of Calcium dependent intercelluar Adhesion Molecule(CAM) whose functions are essential for the induction and maintenance of intercellular cohesion and hepatocyte growth factor(HGF), a potent stimulator of hepatocyte growth, stimulates motility, invasiveness, proliferation, and morphogenesis of epithelium, and also may be involved in tumor invasion. So we examined the role of the E-cadherin/HGF on invasion and metastasis of hypopharyngeal squamous cell carcinoma(SCC). MATERIALS AND METHOD: We performed immunohistochemical stains on 40 tissues of hypopharyngeal cancer patients who were admitted to Ajou University Hospital & Severance Hospital between Mar. 1994 and Dec. 2003 with E-cadherin antibody. We classified the stains according to the intensity and location of E-cadherin expression, and analysis the correlation between the intensity or location of E-cadherin expression and the many clinicopathologic factors. To find the effect of HGF for the E-cadherin expression in the progression of hypopharyngeal cancer, RT-PCR and Western blot in hypopharyngeal cell line(FaDu) were performed according to the concentration of HGF(0, 10, 30 ng/ml). Hypopharyngeal cancer cell line RESULTS: All tissues were positive for the immunohistochemical staining of E-cadherin. According to the intensity +, ++ and +++ group were 25%, 55% and 20% respectively and according to the location membranous and non-membranous group were 50% respectively. Although the intensity of E-cadherin was not correlate with any clinical factor, the location of E-cadherin was siginificantly correlated with lymph node metastasis(p<0.05). Analyze for RT-PCR and Western Blot of E-cadherin expression in FaDu cells revealed the decreased expression of E-cadherin in proportion to the concentration of HGF. CONCLUSION: These results suggest that non-membranous expression of E-cadherin in hypopharyngeal cancer was significantly correlated with lymph node metastasis and that HGF may plays an important role in progression of hypopharyngeal cancer through significantly reducing the expression of E-cadherin.","연구목적: 하인두암은 빠른 증식과 침습적인 성향 및 전이를 잘 하는 특성에 의해 예후가 나쁜 암으로 알려져 있다. 간세포성장인자(Hepatocyte Growth Factor, 이하 HGF)는 간세포 뿐 아니라 체내의 다양한 세포와 종양에서 증식, 분산, 이동 및 침습에 관여하며 종양주위의 신생혈관을 생성하는 것으로 알려져 있고, E-cadherin은 세포 표면에 존재하는 칼슘 의존형 세포 유착물질(Calcium dependent intercelluar Adhesion Molecule, CAM)의 일종으로서, 세포의 골격을 유지하며, 세포간의 유착을 매개하고 조직의 구조를 형성하게 하는 역할을 하고, 종양세포의 E-cadherin은 종양의 침윤을 억제하는 기능이 있는 것으로 알려져 있으며, 양자에 관한 많은 연구가 진행되고 있다. 본 연구는 두경부 종양 중 주변조직으로의 침습과 전이를 잘 하는 하인두암에서 E-cadherin의 발현에 따른 임상적인 의의를 알아보고 하인두암세포주에서 HGF가 E-cadherin의 발현에 어떠한 역할을 하는지를 알아보고자 하였다. 재료 및 방법: 하인두암으로 수술받은 40예의 시료를 이용하여 E-cadherin의 면역조직화학적 염색을 시행하였고 하인두암 세포주인 FaDu(ATCC)을 이용하여 HGF의 농도(10, 30ng/ml)에 따른 E-cadherin의 발현을 보고자RT-PCR과 Western blotting을 시행하였다. 결과: 면역조직화학적 염색상 E-cadherin의 발현정도는 하인두암의 임상양상과 통계적으로 유의한 관계를 보이지 못했으나, E-cadherin의 발현위치는 림프절 전이와 통계적으로 유의한 관계를 보였고(p=0.008), 종양의 재발과도 상당한 관련성(p=0.072)이 의심되었다. 하인두암 세포주의RT-PCR과 Western blotting결과, HGF를 처리한 경우가 대조군에 비해 E-cadherin 발현이 감소하였고 이는 HGF의 농도에 비례하였다. 결론: 하인두암 조직에서E-cadherin의 발현위치가 하인두암의 림프절 전이와 관련이 있으며, 하인두암의 E-cadherin 발현의 감소에HGF가 관여함으로써 하인두암의 침습에 HGF가 일부 영향을 주는 것을 알 수 있었다.","\"국문요약••••••••••••••••••••••••••••••••••••••• i 차례 •••••••••••••••••••••••••••••••••••••••••••• iii 그림 차례 ••••••••••••••••••••••••••••••••••••• v 표 차례 •••••••••••••••••••••••••••••••••••••••• vi Ⅰ. 서론 ••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 1 Ⅱ. 재료 및 방법 •••••••••••••••••••••••••••••••••••••••••••••••• 6 A. 하인두암 환자의 조직을 통한 연구 •••••••••••••••••••• 6 1. 대상 환자 •••••••••••••••••••••••••••••••••••••••••••••••••••••• 6 2. E-cadherin에 대한 면역조직화학적 염색 ••••••••••••• 6 B. 하인두암 세포주를 이용한 연구 ••••••••••••••••••••••••• 7 1. 세포배양 •••••••••••••••••••••••••••••••••••••••••••••••••••••••• 7 2. RT-PCR에 의한 E-cadherin의 mRNA 측정 ••••••••• 8 3. Western blotting을 이용한 E-cadherin 발현 검색 •• 8 C. 통계적 분석 •••••••••••••••••••••••••••••••••••••••••••••••••••• 9 Ⅲ. 결과 •••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 10 A. 하인두암 조직에서 E-cadherin의 발현정도(intensity)와 임상 양상 과의 관계 10 B. 하인두암 조직에서 E-cadherin의 발현위치(location)와 임상 양상 과의 관계• 13 C. 하인두암 세포주에서 RT-PCR을 이용하여 HGF의 농도에 따른 E-cadherin의 mRNA발현 측정 • 17 D. 하인두암 세포주에서 Western blotting을 이용하여 HGF의 농도에 따른 E-cadherin 의 발현 검색 • 18 Ⅳ. 고찰 •••••••••••••••••••••••••••••••••••••••••••• 19 Ⅴ. 결론 •••••••••••••••••••••••••••••••••••••••••••• 24 참고문헌 ••••••••••••••••••••••••••••••••••••••••••• 25 영문요약 ••••••••••••••••••••••••••••••••••••••••••• 34\"","Master"]},{"key":"dc:title","label":"Title","values":["Expression of E-cadherin and HGF in Hypopharyngeal Cancer","하인두암에서 간세포성장인자와 E-cadherin의 발현에 관한 연구"]}]}],"canonical_facts":{"dc:contributor":["김, 철호","대학원 의학과","200324513","이, 장우"],"dc:creator":["이, 장우"],"dc:date":["2011-03-08","2006"],"dc:description":["BACKGROUND AND OBJECTIVES: Head and neck squamous cell carcinoma (HNSCC) results from a multistep carcinogenesis process in which increasing degrees of mucosal changes and cellular atypia occur over large areas of epithelium. Hypopharyngeal squamous cell carcinoma(SCC) is well known as a aggressive cancer which has relatively poor prognosis. E-cadherin is a large family of Calcium dependent intercelluar Adhesion Molecule(CAM) whose functions are essential for the induction and maintenance of intercellular cohesion and hepatocyte growth factor(HGF), a potent stimulator of hepatocyte growth, stimulates motility, invasiveness, proliferation, and morphogenesis of epithelium, and also may be involved in tumor invasion. So we examined the role of the E-cadherin/HGF on invasion and metastasis of hypopharyngeal squamous cell carcinoma(SCC). MATERIALS AND METHOD: We performed immunohistochemical stains on 40 tissues of hypopharyngeal cancer patients who were admitted to Ajou University Hospital & Severance Hospital between Mar. 1994 and Dec. 2003 with E-cadherin antibody. We classified the stains according to the intensity and location of E-cadherin expression, and analysis the correlation between the intensity or location of E-cadherin expression and the many clinicopathologic factors. To find the effect of HGF for the E-cadherin expression in the progression of hypopharyngeal cancer, RT-PCR and Western blot in hypopharyngeal cell line(FaDu) were performed according to the concentration of HGF(0, 10, 30 ng/ml). Hypopharyngeal cancer cell line RESULTS: All tissues were positive for the immunohistochemical staining of E-cadherin. According to the intensity +, ++ and +++ group were 25%, 55% and 20% respectively and according to the location membranous and non-membranous group were 50% respectively. Although the intensity of E-cadherin was not correlate with any clinical factor, the location of E-cadherin was siginificantly correlated with lymph node metastasis(p<0.05). Analyze for RT-PCR and Western Blot of E-cadherin expression in FaDu cells revealed the decreased expression of E-cadherin in proportion to the concentration of HGF. CONCLUSION: These results suggest that non-membranous expression of E-cadherin in hypopharyngeal cancer was significantly correlated with lymph node metastasis and that HGF may plays an important role in progression of hypopharyngeal cancer through significantly reducing the expression of E-cadherin.","연구목적: 하인두암은 빠른 증식과 침습적인 성향 및 전이를 잘 하는 특성에 의해 예후가 나쁜 암으로 알려져 있다. 간세포성장인자(Hepatocyte Growth Factor, 이하 HGF)는 간세포 뿐 아니라 체내의 다양한 세포와 종양에서 증식, 분산, 이동 및 침습에 관여하며 종양주위의 신생혈관을 생성하는 것으로 알려져 있고, E-cadherin은 세포 표면에 존재하는 칼슘 의존형 세포 유착물질(Calcium dependent intercelluar Adhesion Molecule, CAM)의 일종으로서, 세포의 골격을 유지하며, 세포간의 유착을 매개하고 조직의 구조를 형성하게 하는 역할을 하고, 종양세포의 E-cadherin은 종양의 침윤을 억제하는 기능이 있는 것으로 알려져 있으며, 양자에 관한 많은 연구가 진행되고 있다. 본 연구는 두경부 종양 중 주변조직으로의 침습과 전이를 잘 하는 하인두암에서 E-cadherin의 발현에 따른 임상적인 의의를 알아보고 하인두암세포주에서 HGF가 E-cadherin의 발현에 어떠한 역할을 하는지를 알아보고자 하였다. 재료 및 방법: 하인두암으로 수술받은 40예의 시료를 이용하여 E-cadherin의 면역조직화학적 염색을 시행하였고 하인두암 세포주인 FaDu(ATCC)을 이용하여 HGF의 농도(10, 30ng/ml)에 따른 E-cadherin의 발현을 보고자RT-PCR과 Western blotting을 시행하였다. 결과: 면역조직화학적 염색상 E-cadherin의 발현정도는 하인두암의 임상양상과 통계적으로 유의한 관계를 보이지 못했으나, E-cadherin의 발현위치는 림프절 전이와 통계적으로 유의한 관계를 보였고(p=0.008), 종양의 재발과도 상당한 관련성(p=0.072)이 의심되었다. 하인두암 세포주의RT-PCR과 Western blotting결과, HGF를 처리한 경우가 대조군에 비해 E-cadherin 발현이 감소하였고 이는 HGF의 농도에 비례하였다. 결론: 하인두암 조직에서E-cadherin의 발현위치가 하인두암의 림프절 전이와 관련이 있으며, 하인두암의 E-cadherin 발현의 감소에HGF가 관여함으로써 하인두암의 침습에 HGF가 일부 영향을 주는 것을 알 수 있었다.","\"국문요약••••••••••••••••••••••••••••••••••••••• i 차례 •••••••••••••••••••••••••••••••••••••••••••• iii 그림 차례 ••••••••••••••••••••••••••••••••••••• v 표 차례 •••••••••••••••••••••••••••••••••••••••• vi Ⅰ. 서론 ••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 1 Ⅱ. 재료 및 방법 •••••••••••••••••••••••••••••••••••••••••••••••• 6 A. 하인두암 환자의 조직을 통한 연구 •••••••••••••••••••• 6 1. 대상 환자 •••••••••••••••••••••••••••••••••••••••••••••••••••••• 6 2. E-cadherin에 대한 면역조직화학적 염색 ••••••••••••• 6 B. 하인두암 세포주를 이용한 연구 ••••••••••••••••••••••••• 7 1. 세포배양 •••••••••••••••••••••••••••••••••••••••••••••••••••••••• 7 2. RT-PCR에 의한 E-cadherin의 mRNA 측정 ••••••••• 8 3. Western blotting을 이용한 E-cadherin 발현 검색 •• 8 C. 통계적 분석 •••••••••••••••••••••••••••••••••••••••••••••••••••• 9 Ⅲ. 결과 •••••••••••••••••••••••••••••••••••••••••••••••••••••••••••• 10 A. 하인두암 조직에서 E-cadherin의 발현정도(intensity)와 임상 양상 과의 관계 10 B. 하인두암 조직에서 E-cadherin의 발현위치(location)와 임상 양상 과의 관계• 13 C. 하인두암 세포주에서 RT-PCR을 이용하여 HGF의 농도에 따른 E-cadherin의 mRNA발현 측정 • 17 D. 하인두암 세포주에서 Western blotting을 이용하여 HGF의 농도에 따른 E-cadherin 의 발현 검색 • 18 Ⅳ. 고찰 •••••••••••••••••••••••••••••••••••••••••••• 19 Ⅴ. 결론 •••••••••••••••••••••••••••••••••••••••••••• 24 참고문헌 ••••••••••••••••••••••••••••••••••••••••••• 25 영문요약 ••••••••••••••••••••••••••••••••••••••••••• 34\"","Master"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/1577","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001297","000000001297"],"dc:language":["ko"],"dc:subject":["E-cadherin","간세포성장인자","하인두암","침습","전이"],"dc:title":["Expression of E-cadherin and HGF in Hypopharyngeal Cancer","하인두암에서 간세포성장인자와 E-cadherin의 발현에 관한 연구"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:22Z"}