Charakterisierung des allergenen Potentials von Arylaminen : in vitro Studie zur Reaktivität von T-Lymphozyten

Allergic contact dermatitis, a delayed hypersensitivity reaction to small molecular weight compounds (haptens), is a common skin reaction causing personal and occupational problems. Patch tests to para-Phenylenediamine and related substances often reveal concurrent reactions which can be attributed to separate sensitisation or cross-reactivity. In order to understand the health risks associated with cross-reactivity we studied cross-reactivity of different para-amino compounds and arylamines in vitro by measurement of T-cell proliferation of PBMC, T-cell lines and T-cell clones of subjects with a positive patch test result to para-Phenylenediamine. T-cell clones were generated from the blood of 14 cases and their proliferative responses to the chemicals were estimated. Concurrent reactions to these compounds on the polyclonal and monoclonal level were found. A restricted T-cell receptor V beta 16-usage was observed (5/8 clones). A detailed analysis of 38 T-cell clones from four patients showed broad cross-reactivity (64.7%) between para-Phenylenediamine, para-Toluenediamine, Bandrowski’s Base and para-Aminoazobenzene. More restricted patterns were found in 8.8% which responded only to compounds with 2 or 3 benzene rings, whereas 26.5% of the clones reacted specifically only to one compound. More than 60% of the clones showed a broad cross-reactivity pattern. Hence, clinically observed cross-reactivity between different para-amino compounds can be based on a T-cell receptor with low specificity.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2008

Author dc:creator

• Skazik, Claudia

Contributors dc:contributor

• Blömeke, Brunhilde

• info:eu-repo/classification/ddc/570
• T-Lymphozyt
• T-Lymphozyten-Rezeptor
• Kontaktdermatitis
• Hapten
• Arylamine
• Kreuzreaktion
• Biowissenschaften, Biologie
• T-lymphocyte
• allergic contact dermatitis
• cross-reactivity
• arylamines

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112666%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A50109