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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Hapten"”.
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Structure and dynamics of antibody hapten complexes
Antibody molecules are highly antigen-specific receptors of the immune system. In this thesis, structural and functional relationship in antibody molecules are studied.
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Hapten inhibition studies assessing the ability of a thymine derived hapten to bind DNA antibodies in sera from patients with systemic lupus erythematosus (SLE)
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Spectroscopic Studies of Rhodium(ii) Perfluorobutyrate and Synthesis of Metallo-Hapten Complexes
In part I, the bonding interactions between rhodium(II) perfluorobutyrate (Rh(,2)(pfb)(,4)) and several Lewis bases was investigated using electron paramagnetic resonance (EPR) and infrared (IR) spectroscopy. The shift of the g-value in the EPR spectra of the spin label complex …
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Hapten Binding by Lymphocyte Surface Immunoglobulin and the Subsequent Implications for Affinity Maturation
… of the selected for smIg avidity. Fifth, hapten specific T lymphocytes appeared, within the constraints of the reconstitution system, not to influence antibody affinity maturation and therefore, probably did not influence B cell clonal selection and expansion. Carrier specific T …
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The High Affinity Anti-Fluorescyl Immunoglobulin-M Response in Rabbits
… high affinity IgM populations in the fluorescein hapten system. Fluorescein has characteristically elicited IgG antibodies of relatively higher affinity than values observed with other hapten systems. Characterization of protein-ligand interactions, solvent effects and the microenvironment of …
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Kinetics and Intracellular Pathways Required for MHC Ii-Peptide Loading and Surface Expression of a Hapten-Protein Conjugate in Murine Macrophage
… unanswered. Therefore, a novel fluorescent hapten-protein antigen consisting of the small hapten, fluorescein, conjugated to the protein, bovine serum albumin, was constructed. In the globular form, the antigenic probe is relatively non-fluorescent due to the short Stoke's shift of …
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Elucidation of a Macrophage Cell Surface Receptor With Specificity for Exogenous Aromatic Hapten-Protein Antigens and Effects on Antigen Uptake and Processing
… demonstrate the existence of an aromatic hapten-binding plasma membrane protein on macrophage cells with specificity for fluorescein. Moreover, the models proposed in this thesis have helped the further elucidation of the finer principles that dictate exogenous antigen processing and …
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New insights in contact allergy and drug delivery. A study of formulation effects and hapten targets in skin using two-photon fluorescence microscopy
… also showed elevated sensitization potency of haptens depending on delivery vehicles. Putative mechanistic explanations for the observed effects have been proposed. Specifically, phthalates were shown to increase the sensitization potency of isothiocyanates. The phthalate-induced effect could …
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Factors that Affect the Immunogenicity of Lipid-PLGA Nanoparticle-Based Nanovaccines against Nicotine Addiction
… multiple factors, including nanoparticle size, hapten density, hapten localization, carrier protein, and molecular adjuvants, on its immunogenicity has been investigated. Results indicated that all these studied factors significantly affected the immunological efficacy of the nicotine …
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Untersuchungen zur Bindung kontaktallergener Substanzen an nukleophile Aminosäureseitenketten
… einer Kontaktallergie wird die Bildung eines Hapten-Carrier-Komplexes aus dem niedermolekularen Kontaktallergen (Hapten) und Hautproteinen (Carrier) angesehen. Zur Abschätzung der Sensibilisierungsfähigkeit kann daher auch die Reaktivität der Substanz oder ihrer Metaboliten gegenüber Proteinen …
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Elicitation and Characterization of Antibodies With Idiotypic (Ab1) and Anti-Idiotypic (Ab2) Reactivity: Epibodies
… study in which Ab1 and Ab2 responses to the hapten DNP were found to be similar in terms of magnitude and kinetics following immunization. In this study, spleen cells of mice immunized with different dinitrophenylated carriers were fused with myeloma cells to generate monoclonal antibodies. …
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Structure, Function, and Diversity of Monoclonal Anti-Fluorescyl Antibodies
… response were examined using a fluorescein hapten system in conjunction with hybridoma methodology. Monoclonal anti-fluorescyl IgG antibodies (nine clones) were produced by fusion of BALB/c splenocytes and Sp 2/O-Ag 14 myeloma cells. Analyses of these clones were performed in order to …
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Entwicklung neuartiger biomimetischer Sensoren: ein bifunktionaler Sensor auf Basis haptenisierter Cholinesterase
… Zentrum der AChE • ein an die AChE gekoppeltes Hapten und das Epitop eines Antikörpers • ein an die AChE gekoppeltes Hapten, das als Ligand ein weiteres Enzym bindet Neben dem molekularen Erkennungselement wird ein Biosensor ebenso durch die Art des Transducers charakterisiert. Hier werden …
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Catalytic antibodies for amide cleavage and prodrug activation
… to generate amide cleaving antibodies. Two haptens, O1 and O2, were designed and synthesized to create a hydrophobic pocket for an auxiliary nucleophile as well as elicit complementary basic/acidic side chain residues in the antibody combining binding site. The recently established …
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Improved mehtods and reagents for pretargeted radioimmunotherapy of cancer
… window for dosing radionuclide-carrying hapten was found to be strongly dependent on cell-specific parameters such as antigen concentration, void fraction, and the radiosensitivity parameter a, as well as on targeting agent molecular parameters such as the diffusivity and antigen-binding …
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Early divergent B cell differentiation during antibody responses
… in which 5% of B cells are specific for the hapten 4-hydroxy-3-nitrophenol (NP), were immunized with NP-Ficoll. Varying the antigen dose altered the proportion of B cells that entered the extra follicular or GC responses and the amount of class switch recombination (CSR). The expression of …
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Detection of colorectal carcinoma-associated antigens using specific antibodies against human milk oligosaccharides
… were used to detect their homologous haptens as gangliosides in the human colorectal carcinoma cell line SW'lll6. Sialyltetrasaccharide b-ceramide was detected in the monosialylganglioside fraction from human meconium and a total ganglioside fraction from SWlll6 cells on thin layer …
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