Synthesis, Radiolabeling and Evaluation of a suite of Tracers with ⁴⁴Sc for Detecting Extracellular DNA

<p>Neutrophil extracellular traps involve the rapid translocation of DNA to the outside of the cell under certain stimuli. This structure forms a fibrous network that is able to limit the spread of pathogens and to kill microorganisms. It has also been shown to be present in various pathological processes such as inflammation, autoimmune diseases, and cancer metastasis. Currently, the formation process of NETs in vivo is being extensively studied. However noninvasive detection and quantitation has yet to be achieved. A class of PET tracers are described here that consists of a DNA dye as the backbone that is labeled with an emerging positron emitting isotope of research and clinical interest, ⁴⁴Sc. We describe the synthesis and radiolabeling of several NET probes and their in vivo pharmacokinetics.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biomedical Engineering

Year dc:date.available

2023

Author dc:creator

• Li, Zhiyao

Contributors dc:contributor

• Daniel L.J. Thorek, PhD, Radiology, Biomedical Engineering
• Hanwen Zhang, PhD, Radiology Buck Rogers, PhD, Radiation Oncology Michael Nickels, PhD, Radiology

• Positron Emission Tomography
• Neutrophil Extracellular Traps
• Extracellular DNA
• Synthesis
• Radiolabeling
• Pharmacokinetics
• Radiotracer
• Scandium-44
• DNA dye
• Bioimaging and Biomedical Optics
• Organic Chemistry
• Radiochemistry
• Radiology

Statement dc:rights

• I have not registered my thesis with the U.S. Copyright Office, but intend to later.

Language dc:language

English (en)

Identifier

https://openscholarship.wustl.edu/eng_etds/829

OAI identifier oai:identifier

oai:openscholarship.wustl.edu:eng_etds-1891