ADAM10: a Novel Regulator of Mast Cell Function and Activation

<p>In this study we show, to our knowledge, the first description of the role ADAM10 plays on mast cells. ADAM10 is abundantly expressed on mast cells both in vitro and in vivo. Its expression is inhibited by IL-10, a suppressive cytokine. siRNA depletion of ADAM10 on bone marrow-derived mast cells (BMMC) caused decreased IL-6 production following IgE cross-linking and also impaired BMMC stem cell factor (SCF)-induced migration through collagen IV. Mast cells and T helper cells (Th cells) in the peritoneum were reduced in ADAM10 KO mice. In addition, ADAM10 KO BMMC produced significantly less of all cytokines measured following IgE cross-linking, including IL-6, TNF-α, IL-13, and MCP-1, compared to wild type BMMC. Collectively these data show that mast cell ADAM10 can be regulated by a T regulatory cell cytokine, IL-10, and describes key ways in which ADAM10 loss affects prototypical mast cell functions and distribution.</p>

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2012

Author dc:creator

• Faber, Travis

Contributors dc:contributor

• John Ryan

• Mast Cells
• ADAM10
• IgE
• SCF
• Biology
• Life Sciences

Statement dc:rights

• © The Author

Identifier

https://scholarscompass.vcu.edu/etd/354

OAI identifier oai:identifier

oai:scholarscompass.vcu.edu:etd-1353