MT1-MMP enhances cell survival and proliferation via roles unrelated to metabolic transcript levels in MCF7 breast cancer cells

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a multifunctional protease implicated in cancer aggressiveness for its ability to promote proliferation, migration, and invasion, particularly at low expression levels. Due to its multidomain structure and cell signalling abilities, MT1-MMP may act as a key regulatory node enhancing ATP production, which may confer further advantages in cell proliferation and motility. Low levels of MT1-MMP that enhance cell growth/movement were examined with respect to cell metabolism. Cells expressing different levels or forms of MT1-MMP were cultured in media containing different metabolites to assess viability, proliferation, and metabolic gene transcript levels. While cells with a cytoplasmic domain deletion in MT1-MMP demonstrated more transcript level changes than cells that differed in MT1-MMP levels, there were no metabolically relevant differences in metabolic gene transcript levels, suggesting MT1-MMP does not modulate key metabolic pathways to confer proliferative advantages.

Name thesis:degree_name

M Sc

Discipline thesis:degree_discipline

Biology

Grantor dc:publisher

The University of Western Ontario

Year dc:date.issued

2022

Author dc:creator

• Wise, Rachel A

Advisor dc:contributor.advisor

• Damjanovski, Sashko

• Matrix metalloproteinase (MMP)
• Membrane-type 1 matrix metalloproteinase (MT1-MMP)
• MCF7
• Metabolism
• Warburg Effect
• Hypoxia-inducible factor 1-alpha (HIF-1a)

Language dc:language.iso

en_ca

Handle dc:identifier.uri

https://hdl.handle.net/20.500.14721/31919

OAI identifier oai:identifier

oai:uwo.scholaris.ca:20.500.14721/31919