{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/31919"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/31919","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"MT1-MMP enhances cell survival and proliferation via roles unrelated to metabolic transcript levels in MCF7 breast cancer cells","abstract":"Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a multifunctional protease implicated in cancer aggressiveness for its ability to promote proliferation, migration, and invasion, particularly at low expression levels. Due to its multidomain structure and cell signalling abilities, MT1-MMP may act as a key regulatory node enhancing ATP production, which may confer further advantages in cell proliferation and motility. Low levels of MT1-MMP that enhance cell growth/movement were examined with respect to cell metabolism. Cells expressing different levels or forms of MT1-MMP were cultured in media containing different metabolites to assess viability, proliferation, and metabolic gene transcript levels. While cells with a cytoplasmic domain deletion in MT1-MMP demonstrated more transcript level changes than cells that differed in MT1-MMP levels, there were no metabolically relevant differences in metabolic gene transcript levels, suggesting MT1-MMP does not modulate key metabolic pathways to confer proliferative advantages.","abstract_html":"Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a multifunctional protease implicated in cancer aggressiveness for its ability to promote proliferation, migration, and invasion, particularly at low expression levels. Due to its multidomain structure and cell signalling abilities, MT1-MMP may act as a key regulatory node enhancing ATP production, which may confer further advantages in cell proliferation and motility. Low levels of MT1-MMP that enhance cell growth/movement were examined with respect to cell metabolism. Cells expressing different levels or forms of MT1-MMP were cultured in media containing different metabolites to assess viability, proliferation, and metabolic gene transcript levels. While cells with a cytoplasmic domain deletion in MT1-MMP demonstrated more transcript level changes than cells that differed in MT1-MMP levels, there were no metabolically relevant differences in metabolic gene transcript levels, suggesting MT1-MMP does not modulate key metabolic pathways to confer proliferative advantages.","abstract_has_math":false,"creators":["Wise, Rachel A"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Damjanovski, Sashko"],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-02-07","date_published":"2022-02-07","updated_at":"2026-07-27T21:56:01Z","subjects":["Matrix metalloproteinase (MMP)","Membrane-type 1 matrix metalloproteinase (MT1-MMP)","MCF7","Metabolism","Warburg Effect","Hypoxia-inducible factor 1-alpha (HIF-1a)"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/31919","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Damjanovski, Sashko"]},{"key":"dc:creator","label":"Author","values":["Wise, Rachel A"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T19:24:20Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T19:24:20Z"]},{"key":"dc:date.issued","label":"Date","values":["2022-02-07"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Matrix metalloproteinase (MMP)","Membrane-type 1 matrix metalloproteinase (MT1-MMP)","MCF7","Metabolism","Warburg Effect","Hypoxia-inducible factor 1-alpha (HIF-1a)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/31919"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Collaborative Specialization: Developmental Biology","The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a multifunctional protease implicated in cancer aggressiveness for its ability to promote proliferation, migration, and invasion, particularly at low expression levels. Due to its multidomain structure and cell signalling abilities, MT1-MMP may act as a key regulatory node enhancing ATP production, which may confer further advantages in cell proliferation and motility. Low levels of MT1-MMP that enhance cell growth/movement were examined with respect to cell metabolism. Cells expressing different levels or forms of MT1-MMP were cultured in media containing different metabolites to assess viability, proliferation, and metabolic gene transcript levels. While cells with a cytoplasmic domain deletion in MT1-MMP demonstrated more transcript level changes than cells that differed in MT1-MMP levels, there were no metabolically relevant differences in metabolic gene transcript levels, suggesting MT1-MMP does not modulate key metabolic pathways to confer proliferative advantages."]},{"key":"dc:title","label":"Title","values":["MT1-MMP enhances cell survival and proliferation via roles unrelated to metabolic transcript levels in MCF7 breast cancer cells"]}]}],"canonical_facts":{"dc:contributor.advisor":["Damjanovski, Sashko"],"dc:creator":["Wise, Rachel A"],"dc:date.accessioned":["2025-07-10T19:24:20Z"],"dc:date.available":["2025-07-10T19:24:20Z"],"dc:date.issued":["2022-02-07"],"dc:description":["Collaborative Specialization: Developmental Biology","The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a multifunctional protease implicated in cancer aggressiveness for its ability to promote proliferation, migration, and invasion, particularly at low expression levels. Due to its multidomain structure and cell signalling abilities, MT1-MMP may act as a key regulatory node enhancing ATP production, which may confer further advantages in cell proliferation and motility. Low levels of MT1-MMP that enhance cell growth/movement were examined with respect to cell metabolism. Cells expressing different levels or forms of MT1-MMP were cultured in media containing different metabolites to assess viability, proliferation, and metabolic gene transcript levels. While cells with a cytoplasmic domain deletion in MT1-MMP demonstrated more transcript level changes than cells that differed in MT1-MMP levels, there were no metabolically relevant differences in metabolic gene transcript levels, suggesting MT1-MMP does not modulate key metabolic pathways to confer proliferative advantages."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/31919"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Matrix metalloproteinase (MMP)","Membrane-type 1 matrix metalloproteinase (MT1-MMP)","MCF7","Metabolism","Warburg Effect","Hypoxia-inducible factor 1-alpha (HIF-1a)"],"dc:title":["MT1-MMP enhances cell survival and proliferation via roles unrelated to metabolic transcript levels in MCF7 breast cancer cells"],"dc:type":["thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:56:01Z"}