Effects of Leucine on Skeletal Muscle During 14 d Bed Rest in Middle-aged Adults

Aging is associated with a slow, progressive loss of muscle mass and strength. Mechanical unloading, such as that commonly experienced during hospitalization or spaceflight, results in a rapid loss of muscle mass and strength, particularly in older adults. Exercise, a potent countermeasure to such losses, is often impractical in acutely ill patients. The essential amino acid leucine has been shown to acutely stimulate muscle protein synthesis (MPS), a decrease in which mechanistically drives inactivity-induced losses in muscle. This study evaluated the effects of leucine supplementation (0.06 g • kg • meal-1; LEU) with each of three daily meals in middle-aged adults, a largely unstudied age group, during 14 d bed rest (BR) and subsequent 7 d rehabilitation. Primary findings were: 1) leucine attenuated the loss of whole body lean mass during the first 7 d of BR compared to control subjects (LEU: -0.6±0.2 kg vs. CON: -1.1±0.2 kg, p<0.05) and reduced or prevented decrements in knee extensor strength (LEU: -8±3% vs. CON: -15±3%, p<0.05), ankle extensor strength (LEU: -13±5% vs. CON: -20±5%, p<0.05), and knee extensor endurance (LEU: -2±4% vs. CON: -14±3%, p<0.05) during 14 d BR; 2) LEU maintained both post-absorptive and post-prandial MPS during BR; in contrast, BR decreased post-absorptive MPS (pre-BR: 0.061% • h-1 vs. post-BR: 0.043% • h-1, p<0.05); 3) insulin area under the curve during an oral glucose tolerance test was unchanged in LEU after BR (21±8%) but elevated in CON (52±23%, p<0.05) and whole body insulin sensitivity in LEU was significantly increased above pre-BR values after 7 d rehabilitation (17±10% vs. CON: -9±9%, p<0.05). Leucine is an inexpensive, low volume supplement that can be easily incorporated into the daily meals of middle-aged adults to maintain muscle protein synthesis and protect muscle mass, strength, and insulin sensitivity during periods of physical inactivity characteristic of hospitalized acute illness and spaceflight.

Name thesis:degree_name

Rehabilitation Science

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Nutrition and Metabolism

Grantor

The University of Texas Medical Branch at Galveston

Author dc:creator

• English, Kirk L

Advisor dc:contributor.advisor

• Paddon-Jones, Douglas J

Committee members dc:contributor.committeemember

• Adams, Gregory R.
• Ploutz-Snyder, Robert
• Sheffield-Moore, Melinda
• Sidossis, Labros S.

• Bed rest
• atrophy
• muscle metabolism
• protein synthesis
• muscle mass
• muscle strength
• insulin sensitivity
• leucine
• essential amino acid
• nutrition
• aging
• middle-aged
• stable isotope tracer
• FSR
• mTOR
• spaceflight

Handle dc:identifier.uri

http://hdl.handle.net/2152.3/666

OAI identifier oai:identifier

oai:utmb-ir.tdl.org:2152.3/666