Peptide Vaccine Formulation Controls The Duration of Antigen Presentation and Magnitude of Tumor‐Specific Cd8+ T Cell Response

<p>Despite remarkable progresses in vaccinology, therapeutic cancer vaccines have not achieved their full potential. We previously showed that the duration of antigen presentation critically affected the quantity and quality of T cell response and subsequent anti‐tumor efficacy. Here we describe L‐tyrosine amino acid‐based microparticles as a novel peptide vaccine adjuvant for the induction of tumor‐specific T cells. L‐tyrosine microparticles did not induce inflammasome activation, but instead extended antigen presentation time. The consequent prolongation in antigen presentation translated into prolonged T cell proliferation and superior numbers and anti‐tumor function of vaccination‐induced CD8+ T cells. Indeed, prolonging antigen presentation by repeated injection of peptide in saline resulted in an increase in T cell numbers similar to that observed after vaccination with peptide/L‐tyrosine microparticles. These results suggest that the duration of antigen presentation is critical for optimal induction of anti‐tumor T cells, and can be manipulated through proper vaccine formulation.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation (PhD)

Year dc:date.available

2017

Authors dc:creator

• Khong, Hiep
• <p>0000-0003-4888-7862</p>

Contributors dc:contributor

• Willem W. Overwijk
• Gregory A. Lizee
• Pierre D. McCrea

• adjuvant
• CD8 T cell
• cancer
• vaccine
• pmel-1
• B16 melanoma
• L-tyrosine
• Immunology and Infectious Disease
• Medicine and Health Sciences

Repository record dc:identifier

https://digitalcommons.library.tmc.edu/utgsbs_dissertations/773

OAI identifier oai:identifier

oai:digitalcommons.library.tmc.edu:utgsbs_dissertations-1815