Biochemical Assay Optimization and Computational Screening Efforts to Identify Potential LuxS Inhibitors

<p>Quorum sensing (QS) is a process of coordination of bacterial gene expression in response to cell population. System two QS is regulated by the small signaling molecule autoinducer-2 (AI-2) and is implicated in the infectious behaviors of various bacterial species. AI-2 is biosynthesized from <em>S-</em>ribosylhomocysteine (SRH) by the enzyme LuxS and induces interspecies cell-to-cell communication. Inhibition of LuxS would therefore inhibit interspecies QS. Herein, a search for novel molecular species that will competitively bind with SRH in the LuxS binding site is performed <em>in silico</em>. Computational screening results are then validated <em>in vitro</em> using an optimized LuxS inhibition assay.</p>

Name thesis:degree_name

Master of Science in Chemistry

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Chemistry

Year dc:date.available

2013

Author dc:creator

• Wang, Keeshia Q

Contributors dc:contributor

• Dr. Megan E. Bolitho
• Dr. Lawrence Margerum
• Dr. Jie Jack Li

• LuxS
• Quorum Sensing
• Arginine
• Ellman's Assay
• Docking
• Inhibitor
• Amino Acids, Peptides, and Proteins
• Biochemical Phenomena, Metabolism, and Nutrition
• Biochemistry
• Biotechnology
• Enzymes and Coenzymes
• Medical Biochemistry
• Other Biochemistry, Biophysics, and Structural Biology
• Other Chemicals and Drugs
• Pharmaceutical Preparations

Repository record dc:identifier

https://repository.usfca.edu/thes/149

OAI identifier oai:identifier

oai:repository.usfca.edu:thes-1168