Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit

A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) lever arm orientations. The last acceptor allows the detection of S1's bound near or in between troponin complexes on the thin filament. Additionally, calcium related changes between troponin T and myosin were detected. Based on this data, the troponin complex situated every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas myosins bound in between troponin complexes undergo complete power strokes.

Grantor dc:publisher

University of North Texas

Year dc:date

2007

Author dc:creator

• Coffee Castro-Zena, Pilar G.

Contributors dc:contributor

• Root, Douglas D.
• Chapman, Kent D.
• Dickstein, Rebecca

• luminescent resonance energy transfer
• LRET
• troponin
• acceptors
• Myosin.
• Energy transfer.
• Muscles -- Molecular aspects.
• Muscles -- Cytochemistry.

Statement dc:rights

• Public
• Copyright
• Coffee Castro-Zena, Pilar G.
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 174243727
https://digital.library.unt.edu/ark:/67531/metadc3688/
ark: ark:/67531/metadc3688

OAI identifier oai:identifier

info:ark/67531/metadc3688