{"id":{"repo_id":"unt","oai_identifier":"info:ark/67531/metadc3688"},"canonical_url":"https://search.dev.ndltd.org/etd/unt/info:ark/67531/metadc3688","repository":{"repo_id":"unt","name":"University of North Texas","base_url":"https://digital.library.unt.edu/oai/"},"display":{"title":"Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit","abstract":"A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) lever arm orientations. The last acceptor allows the detection of S1's bound near or in between troponin complexes on the thin filament. Additionally, calcium related changes between troponin T and myosin were detected. Based on this data, the troponin complex situated every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas myosins bound in between troponin complexes undergo complete power strokes.","abstract_html":"A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) lever arm orientations. The last acceptor allows the detection of S1's bound near or in between troponin complexes on the thin filament. Additionally, calcium related changes between troponin T and myosin were detected. Based on this data, the troponin complex situated every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas myosins bound in between troponin complexes undergo complete power strokes.","abstract_has_math":false,"creators":["Coffee Castro-Zena, Pilar G."],"institution":"University of North Texas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Root, Douglas D.","Chapman, Kent D.","Dickstein, Rebecca"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007-05","date_published":"2007-05","updated_at":"2026-07-24T05:34:52Z","subjects":["luminescent resonance energy transfer","LRET","troponin","acceptors","Myosin.","Energy transfer.","Muscles -- Molecular aspects.","Muscles -- Cytochemistry."],"languages":["English"],"rights":["Public","Copyright","Coffee Castro-Zena, Pilar G.","Copyright is held by the author, unless otherwise noted. All rights reserved."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oclc: 174243727","https://digital.library.unt.edu/ark:/67531/metadc3688/","ark: ark:/67531/metadc3688"],"render_values":[{"text":"oclc: 174243727","href":null,"code":true},{"text":"https://digital.library.unt.edu/ark:/67531/metadc3688/","href":"https://digital.library.unt.edu/ark:/67531/metadc3688/","code":true},{"text":"ark: ark:/67531/metadc3688","href":null,"code":true}]}]},"links":{"outbound_url":"https://doi.org/10.12794/metadc3688","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Root, Douglas D.","Chapman, Kent D.","Dickstein, Rebecca"]},{"key":"dc:creator","label":"Author","values":["Coffee Castro-Zena, Pilar G."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2007-05"]},{"key":"dc:publisher","label":"Institution","values":["University of North Texas"]},{"key":"dc:type","label":"Dc Type","values":["Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["luminescent resonance energy transfer","LRET","troponin","acceptors","Myosin.","Energy transfer.","Muscles -- Molecular aspects.","Muscles -- Cytochemistry."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["Public","Copyright","Coffee Castro-Zena, Pilar G.","Copyright is held by the author, unless otherwise noted. 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Based on this data, the troponin complex situated every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas myosins bound in between troponin complexes undergo complete power strokes."]},{"key":"dc:format","label":"Dc Format","values":["Text"]},{"key":"dc:title","label":"Title","values":["Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit"]}]}],"canonical_facts":{"dc:contributor":["Root, Douglas D.","Chapman, Kent D.","Dickstein, Rebecca"],"dc:creator":["Coffee Castro-Zena, Pilar G."],"dc:date":["2007-05"],"dc:description":["A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) lever arm orientations. The last acceptor allows the detection of S1's bound near or in between troponin complexes on the thin filament. Additionally, calcium related changes between troponin T and myosin were detected. Based on this data, the troponin complex situated every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas myosins bound in between troponin complexes undergo complete power strokes."],"dc:format":["Text"],"dc:identifier":["oclc: 174243727","doi: 10.12794/metadc3688","https://digital.library.unt.edu/ark:/67531/metadc3688/","ark: ark:/67531/metadc3688"],"dc:language":["English"],"dc:publisher":["University of North Texas"],"dc:rights":["Public","Copyright","Coffee Castro-Zena, Pilar G.","Copyright is held by the author, unless otherwise noted. All rights reserved."],"dc:subject":["luminescent resonance energy transfer","LRET","troponin","acceptors","Myosin.","Energy transfer.","Muscles -- Molecular aspects.","Muscles -- Cytochemistry."],"dc:title":["Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit"],"dc:type":["Thesis or Dissertation"]},"updated_at":"2026-07-24T05:34:52Z"}