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Showing 1 to 16 of 16 for “"LRET"”.
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Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit
A novel luminescence resonance energy transfer (LRET) nanocircuit assay involving a donor and two acceptors in tandem was developed to study the dynamic interaction of skeletal muscle contraction proteins. The donor transmits energy relayed to the acceptors distinguishing myosin subfragment-1 (S1) …
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Studies of myosin mechanics using fluorescence
… Lanthanide based resonance energy transfer (LRET), fluorescence imaging with one nanometer accuracy (FIONA), and defocused orientation and position imaging (DOPI) are all applied to specifically myosin II and myosin VI in the text below. FRET or a modified version known as LRET, allow for the …
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Conformational Changes In The Extracellular Domain of Glutamate Receptors
… called Luminescence Resonance Energy Transfer (LRET). LRET offers the unique advantage of tracking large scale conformational changes associated with receptor activation and desensitization. In this dissertation, LRET, in combination with biochemical and electrophysiological studies, were …
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Luminescence Resonance Energy Transfer Studies of the Shaker Potassium Voltage-Gated Ion Channel
… called Lanthanide Resonance Energy Transfer (LRET), which gives an accurate distance measurement between two positions on the ion channel. We use two different configurations for LRET on Shaker. The first measures distances between the four identical S4 amino-acid sites on the homotetrameric …
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Ampa Receptor Allosterism: Measurement of The Conformational Changes In The Ligand Binding Domain of A Functional Receptor
… correlates with extent of activation. Here, LRET has been used to show that a similar relationship between cleft closure and activation is observed in the “full length” receptor showing that the isolated ligand binding domain is a good model of the domain in the full length receptor for …
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Novel Lanthanide Containing Polymers for Nucleic Acid Delivery and Monitoring of Polyplex Dynamics
… using lanthanide resonance energy transfer (LRET). Polyplexes were formulated with Tb chelated N5 polymer and tetramethyl rhodamine (TMR) labeled pDNA, which are "LRET pairs". We observed decrease in luminescence intensity of Tb polymer (donor) in close proximity of TMR DNA (acceptor) in an …
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Investigations of The Structure-Function Relationship In Kainate Receptors Using FöRster Resonance Energy Transfer
… utilizes luminescence resonance energy transfer (LRET) and single-molecule Förster Resonance Energy Transfer (smFRET) in order to address this gap in the knowledge. We have characterized the structural arrangement and dynamics of the homomeric (GluK2) receptors and identified structural changes …
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Analysis of the peroxidase-catalysed oxidation of hydroxamic acids
… followed by long-range electron-transfer (LRET) to the active site (i.e. Phe68 in HRP; Pro115/Ala114→sheet A→helix 2 or Pro424→sheet E→helix 13 in LPO). Peroxidases are likely to be partially responsible for the inactivation of hydroxamic acid-based drugs in vivo. These data reported here …
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The Structural Mechanism of Allosteric Modulation of The Nmda Receptor: A Balance of Tensions
… of the receptor using electrophysiology. LRET measurements were made between subunits to determine the architecture of the ATDs in an assembled receptor.</p> <p>Our studies reveal that the ATDs have a compact overall organization, and that the inherent conformation of a GluN1 or GluN2 ATD …
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Development of Tyrosine Kinase Peptide Biosensors and Methods for Detection
… resonance energy transfer (TR-LRET) between the terbium bound phosphopeptides and the orthogonal fluorophore labels.</p>
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Biochemical and biophysical investigations of N-linked glycosylation pathways in archaea
… dynamics of AglB. An intramolecular LRET experimental system was developed to report on substrate binding and the resulting structural transformations in AgIB. There is a strong need for detailed studies on the mechanistic and functional significance of archaeal adaptations of …
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Polyprenyl-dependent glycan assembly pathways in microbial pathogens
… described toward the development of FRET- and LRET-based methodology for future characterization of the protein-protein interactions between PglC and PglA. This work establishes a basis for future studies of polyprenols in the C. jejuni glycan assembly process and may be applicable to other …
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Digital Profiling of Circulating Extracellular Vesicles at Single-Upconversion Nanoparticle Sensitivity and Resolution
… previous luminescence resonance energy transfer (LRET) method using UCNPs for detection of EVs, our technique achieves single tumour-derived sEV quantification. In chapter 3, we report super-resolution imaging technique for single CD9+EpCAM+EV analysis. The upconversion luminescence of single …
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Luminescence resonance energy transfer studies of the Shaker K+ voltage-gated ion channel
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-26T23:06:21Z No. of bitstreams: 1 Posson_David.pdf: 7844031 bytes, checksum: 3cee2902577cf067066066ebcaad3557 (MD5)
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Novel peptide-based approaches to study the activity and substrate specificity of protein kinases
… by Luminescence Resonance Energy Transfer (LRET). This method could be used to monitor both the activity of a tyrosine kinase (SFK) as well as a serine kinase (cAMP-dependent protein kinase; PKA), although the limited water-solubility of the europium complex required the use of 20% DMSO …
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Swarna Ramaswamy_Thesis
<p>STRUCTURAL INVESTIGATIONS OF LIGAND GATED ION CHANNELS</p> <p>Swarna Ramaswamy, B.S</p> <p>Advisor: Vasanthi Jayaraman, Ph.D.</p> <p>Ion channels form an integral part of membrane proteins. In the nervous system including the central and the peripheral nervous system, ligand gated ion channels …