Head Trauma Release of Histamine from Dural Mast Cells Alters Blood-Brain Barrier: Attenuation with Zolantidine

This study employed a new model of mild-to-moderate head trauma to specifically identify the role of dural mast cell (MC) histamine in trauma-induced increased permeability in the blood-brain barrier (BBB). A single line was scored partially through the left dorsal parietal skull. Immediately following the trauma, degranulation was seen in 39% of the MCs on the left and in 2% on the right. After a 20 min survival period, left duras showed 55% with MC degranulation (fewer with complete degranulation) compared to 34% on the right. In the other experiments two parallel lines were scored following the injection of Evan's blue. Histamine assay showed histamine increased in the left cortex to 154% at 5 min, 174% at 10 min, and 151% at 20 min. Fluorescent quantitation of extravasated Evan's blue at 20 min following the trauma gave an increase of 1385% over the value measured for the right cortex. Zolantidine, a selective histamine H2 receptor antagonist, administered at 10- and 20- mg/kg 30 min before the trauma blocked 65% of the Evan's blue extravasation compared with the control and 2.5 mg group.

Grantor dc:publisher

University of North Texas

Year dc:date

2000

Author dc:creator

• Laufer, Susan R.

Contributors dc:contributor

• O'Donovan, Gerard A.
• Orr, Edward
• Droge, Michael
• Kelly, Kimberly S.
• Goven, Arthur J.

• Brain -- Wounds and injuries.
• Histamine.
• Blood-brain barrier.
• head trauma
• blood-brain barrier
• BBB
• histamine
• MC degranulation

Statement dc:rights

• Public
• Copyright
• Laufer, Susan R.
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 47881769
https://digital.library.unt.edu/ark:/67531/metadc2714/
ark: ark:/67531/metadc2714

OAI identifier oai:identifier

info:ark/67531/metadc2714