{"id":{"repo_id":"unt","oai_identifier":"info:ark/67531/metadc2714"},"canonical_url":"https://search.dev.ndltd.org/etd/unt/info:ark/67531/metadc2714","repository":{"repo_id":"unt","name":"University of North Texas","base_url":"https://digital.library.unt.edu/oai/"},"display":{"title":"Head Trauma Release of Histamine from Dural Mast Cells Alters Blood-Brain Barrier: Attenuation with Zolantidine","abstract":"This study employed a new model of mild-to-moderate head trauma to specifically identify the role of dural mast cell (MC) histamine in trauma-induced increased permeability in the blood-brain barrier (BBB). A single line was scored partially through the left dorsal parietal skull. Immediately following the trauma, degranulation was seen in 39% of the MCs on the left and in 2% on the right. After a 20 min survival period, left duras showed 55% with MC degranulation (fewer with complete degranulation) compared to 34% on the right. In the other experiments two parallel lines were scored following the injection of Evan's blue. Histamine assay showed histamine increased in the left cortex to 154% at 5 min, 174% at 10 min, and 151% at 20 min. Fluorescent quantitation of extravasated Evan's blue at 20 min following the trauma gave an increase of 1385% over the value measured for the right cortex. Zolantidine, a selective histamine H2 receptor antagonist, administered at 10- and 20- mg/kg 30 min before the trauma blocked 65% of the Evan's blue extravasation compared with the control and 2.5 mg group.","abstract_html":"This study employed a new model of mild-to-moderate head trauma to specifically identify the role of dural mast cell (MC) histamine in trauma-induced increased permeability in the blood-brain barrier (BBB). A single line was scored partially through the left dorsal parietal skull. Immediately following the trauma, degranulation was seen in 39% of the MCs on the left and in 2% on the right. After a 20 min survival period, left duras showed 55% with MC degranulation (fewer with complete degranulation) compared to 34% on the right. In the other experiments two parallel lines were scored following the injection of Evan's blue. Histamine assay showed histamine increased in the left cortex to 154% at 5 min, 174% at 10 min, and 151% at 20 min. Fluorescent quantitation of extravasated Evan's blue at 20 min following the trauma gave an increase of 1385% over the value measured for the right cortex. Zolantidine, a selective histamine H2 receptor antagonist, administered at 10- and 20- mg/kg 30 min before the trauma blocked 65% of the Evan's blue extravasation compared with the control and 2.5 mg group.","abstract_has_math":false,"creators":["Laufer, Susan R."],"institution":"University of North Texas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["O'Donovan, Gerard A.","Orr, Edward","Droge, Michael","Kelly, Kimberly S.","Goven, Arthur J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2000,"date_issued":"2000-12","date_published":"2000-12","updated_at":"2026-07-24T05:34:52Z","subjects":["Brain -- Wounds and injuries.","Histamine.","Blood-brain barrier.","head trauma","blood-brain barrier","BBB","histamine","MC degranulation"],"languages":["English"],"rights":["Public","Copyright","Laufer, Susan R.","Copyright is held by the author, unless otherwise noted. All rights reserved."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oclc: 47881769","https://digital.library.unt.edu/ark:/67531/metadc2714/","ark: ark:/67531/metadc2714"],"render_values":[{"text":"oclc: 47881769","href":null,"code":true},{"text":"https://digital.library.unt.edu/ark:/67531/metadc2714/","href":"https://digital.library.unt.edu/ark:/67531/metadc2714/","code":true},{"text":"ark: ark:/67531/metadc2714","href":null,"code":true}]}]},"links":{"outbound_url":"https://doi.org/10.12794/metadc2714","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["O'Donovan, Gerard A.","Orr, Edward","Droge, Michael","Kelly, Kimberly S.","Goven, Arthur J."]},{"key":"dc:creator","label":"Author","values":["Laufer, Susan R."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2000-12"]},{"key":"dc:publisher","label":"Institution","values":["University of North Texas"]},{"key":"dc:type","label":"Dc Type","values":["Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Brain -- Wounds and injuries.","Histamine.","Blood-brain barrier.","head trauma","blood-brain barrier","BBB","histamine","MC degranulation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["Public","Copyright","Laufer, Susan R.","Copyright is held by the author, unless otherwise noted. 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In the other experiments two parallel lines were scored following the injection of Evan's blue. Histamine assay showed histamine increased in the left cortex to 154% at 5 min, 174% at 10 min, and 151% at 20 min. Fluorescent quantitation of extravasated Evan's blue at 20 min following the trauma gave an increase of 1385% over the value measured for the right cortex. Zolantidine, a selective histamine H2 receptor antagonist, administered at 10- and 20- mg/kg 30 min before the trauma blocked 65% of the Evan's blue extravasation compared with the control and 2.5 mg group."]},{"key":"dc:format","label":"Dc Format","values":["Text"]},{"key":"dc:title","label":"Title","values":["Head Trauma Release of Histamine from Dural Mast Cells Alters Blood-Brain Barrier: Attenuation with Zolantidine"]}]}],"canonical_facts":{"dc:contributor":["O'Donovan, Gerard A.","Orr, Edward","Droge, Michael","Kelly, Kimberly S.","Goven, Arthur J."],"dc:creator":["Laufer, Susan R."],"dc:date":["2000-12"],"dc:description":["This study employed a new model of mild-to-moderate head trauma to specifically identify the role of dural mast cell (MC) histamine in trauma-induced increased permeability in the blood-brain barrier (BBB). A single line was scored partially through the left dorsal parietal skull. Immediately following the trauma, degranulation was seen in 39% of the MCs on the left and in 2% on the right. After a 20 min survival period, left duras showed 55% with MC degranulation (fewer with complete degranulation) compared to 34% on the right. In the other experiments two parallel lines were scored following the injection of Evan's blue. Histamine assay showed histamine increased in the left cortex to 154% at 5 min, 174% at 10 min, and 151% at 20 min. Fluorescent quantitation of extravasated Evan's blue at 20 min following the trauma gave an increase of 1385% over the value measured for the right cortex. Zolantidine, a selective histamine H2 receptor antagonist, administered at 10- and 20- mg/kg 30 min before the trauma blocked 65% of the Evan's blue extravasation compared with the control and 2.5 mg group."],"dc:format":["Text"],"dc:identifier":["oclc: 47881769","doi: 10.12794/metadc2714","https://digital.library.unt.edu/ark:/67531/metadc2714/","ark: ark:/67531/metadc2714"],"dc:language":["English"],"dc:publisher":["University of North Texas"],"dc:rights":["Public","Copyright","Laufer, Susan R.","Copyright is held by the author, unless otherwise noted. All rights reserved."],"dc:subject":["Brain -- Wounds and injuries.","Histamine.","Blood-brain barrier.","head trauma","blood-brain barrier","BBB","histamine","MC degranulation"],"dc:title":["Head Trauma Release of Histamine from Dural Mast Cells Alters Blood-Brain Barrier: Attenuation with Zolantidine"],"dc:type":["Thesis or Dissertation"]},"updated_at":"2026-07-24T05:34:52Z"}