Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 78 for “"Degranulation"”.
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Modulation of neutrophil degranulation by hypoxia
… stimuli induce minimal extracellular degranulation. Sites of inflammation and infection are usually hypoxic, and it has been shown that oxygen depletion compromises neutrophil function by impairing the generation of reactive oxygen species and hence bacterial killing. The key finding …
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Reconstitution of Munc18-Dependent Mast Cell Degranulation
… of each of these Munc18 isoforms in mast cell degranulation have not been clearly defined. In this study we investigated the functional relationship between Munc18 and eight sets of degranulation-relevant SNAREs using <em>in vitro</em> reconstitution. We showed that Munc18a was active in …
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Stereoselektive Synthese von Sphingolipiden zur Inhibierung der Degranulation von Mastzellen
Die Degranulation von Mastzellen soll durch Glycosphingolipide, welche mit der Zellmembran wechselwirken inhibiert werden. Der Sphingosingrundkörper wurde in zehn-stufigen Synthese ausgehend von N-Boc-Serin, aufgebaut. Die anschließende Glycosylierung erfolgte nach der Trichloracetimidatmethode in …
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Hypoxia induces selective neutrophil degranulation to promote endothelial damage in COPD
Neutrophils are the key effector cells of innate immunity, rapidly recruited to defend the host against invading pathogens. They deploy pre-formed proteases packaged within cytoplasmic granules, generate reactive oxygen species and release neutrophil extracellular traps (NETs). However, these …
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Functional characterization of equine neutrophils in response to calcium ionophore A23187 and phorbol myristate acetate
… gradient technique. Neutrophil aggregation, degranulation, and superoxide anion production were evaluated in assay systems which had previously been established to guantitate PMN function. Dose-response curves for A23187 and PMA were derived for the three functions. Results indicate that …
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Identifying regulators of cytotoxic T cell function through molecular and genetic screening
… from lytic granules. This process is known as degranulation. In this study, I aimed to establish the CRISPR-Cas9 gene editing technology in primary T cells and to optimise screening approaches to identify regulators of CTL killing. The first half of the thesis focuses on primary mouse CTL. The …
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Head Trauma Release of Histamine from Dural Mast Cells Alters Blood-Brain Barrier: Attenuation with Zolantidine
… skull. Immediately following the trauma, degranulation was seen in 39% of the MCs on the left and in 2% on the right. After a 20 min survival period, left duras showed 55% with MC degranulation (fewer with complete degranulation) compared to 34% on the right. In the other experiments two …
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Metabolic Regulation of Mast Cell Regranulation
… to minutes upon activation in a process called degranulation. MCs have beneficial roles in pathogen clearance and wound healing but are most widely associated with their deleterious effects in allergic diseases. Importantly, MCs have been shown to reform granules following degranulation in …
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Effect of phosphorylcholine-based small molecule analogues of the immunomodulatory nematode product ES-62 on mast cell function
… project, it was also shown that ES-62 prevented degranulation of human mast cells by forming a complex with TLR-4 and subsequently sequestering PKC-α away from the high affinity receptor for IgE, FcεRI. In this work, PKC-α was found to be critical for mast cell degranulation and its degradation …
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ROLES OF MAST CELL G PROTEIN-COUPLED RECEPTOR KINASE 2 (GRK2) IN ALLERGY AND INFLAMMATION
… of STAT5, calcium mobilization, and degranulation. GRK2 inhibitors (paroxetine and its analog CCG258747) inhibited FcεRI-induced calcium mobilization and degranulation, and their intravenous administration in mice resulted in substantial reduction of IgE-mediated anaphylaxis. However, …
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Study of cellular mechanisms of inflammation and the involvement of mast cells in disease
… examples wherein CFMA is used to study mast cell degranulation in response to an altered in vivo inflammatory microenvironment, such as the chronic inflammation associated with sickle hemoglobin expression (Chapter 2), chronic in vivo morphine exposure (Chapter 2), and the effects of the …
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Disabled-2 regulates platelet heterotypic and homotypic aggregation through sulfatide binding
… through stimulation of continued platelet degranulation, and these surface receptors mediate platelet heterotypic and homotypic aggregation. Here, we show that Dab2 N-PTB binding of sulfatides serves to increase the inhibitory affect of Dab2. Sulfatide stimulation of platelet degranulation …
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Investigating the negative regulatory role of neurogranin in IgE-mediated mast cell activation
… following mast cell activation is essential for degranulation and pro-inflammatory cytokine/chemokine production, processes that require the Ca2+/calmodulin (CaM)-dependent phosphatase, calcineurin. Neurogranin is a protein that regulates Ca2+-mediated signaling pathways by binding and …
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Natural Killer Cell Responses in Chronic Hepatitis C Virus Infection
… T cells expressing CD107a, a marker of cellular degranulation. Variance between responses in HCV-infected versus healthy donors and variance between NK versus T cell responses were analyzed by matched two-way ANOVA with multiple comparisons and Bonferroni post-test using Graphpad Prism software. …
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Mechanical Deformation of the Extracellular Matrix Mediates Cellular Activation and Mechanotransduction
… and/or 10% peak strain) had on 1) RBL-2H3 cell degranulation; 2) the influences RBL-2H3 cells have on fibroblast function; and 3) age-dependent differences in cardiac fibroblast gene expression. In addition, we examined the differences in ECM remodeling behavior by neonatal and adult cardiac …
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Unraveling the Molecular Mechanisms of Munc18 and Munc13 in Mast Cell Exocytosis
… Munc13-4 plays an essential role in mast cell degranulation; mutational studies revealed that point mutations in C2 domains of Munc13-4 dramatically alter Ca2+-sensitivity of degranulation, whereas absence of Munc13-4 or multiple mutations in C2 domains result in almost complete loss of …
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The Role of MRGPRX2/Mrgprb2 in Regulating Mast Cell Function
… Calcium" signaling" is" essential" for" MC" degranulation" and" function." In" this" dissertation" we" present" an" evidence" that" STIM1/Orai1" plays" an" important" role" in" MRGPRX2" mediated" calcium" influx" and" mediators"release"when"stimulated"with"LLE37"and"SP."" …
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MRGPRX2 Signaling on Mast Cell-Mediated Pseudoallergy and Neuroimmune Interaction
… also tested. We found that rocuronium induced degranulation in murine and human MCs via MrgprB2 and MRGPRX2, respectively, but with different affinities, indicating important functional differences between these receptors. This indicates that mice expressing MrgprB2 may not be a suitable model …
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Molecular basis of Fce receptor: IgE interactions
… a cascade of events resulting in cellular degranulation and the release of inflammatory mediators. Many present treatments for these conditions act at this stage to inhibit the activity of these chemical intermediaries, after inflammation has been initiated. This thesis specifically …
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Host Defense Mechanisms in the Crayfish: the Effect of Injection with Live or Killed Bacteria.
… of receptor bearing hemocytes may be due to the degranulation of the receptor bearing hemocytes.</p>
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