Transcriptiome analysis of a migraine model

Migraine is a serious episodic headaches affecting more than 15% of the human population with a detrimental influence on the patients’ life and socioeconomic functioning. Although many pathophysiological and molecular mechanisms of migraine have been suggested, little is known about the molecular disruption in a chronic migraine. Here, we propose to characterize the differences of the gene expression, biological processes and pathways, and regulatory network between individuals with control and a hyperalgesia mouse model of chronic migraine. Chronic hyperalgesia was modeled using a nitroglycerin treatment. The transcriptome was characterized in the trigeminal ganglia and nucleus accumbens. Samples were sequenced using the Illumina HiSeq 4000 platform to produce paired end reads of 100 bp. Opalin, Slc32a, Cacna1b, and H2-Eb2 were among the 110 genes exhibiting significant interaction effects. The 165 genes presenting different expression patterns between the treatment and control groups included Aldh1a1 and Slc7a2. A comparative transcriptomic analysis offered insights for migraine therapies.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Animal Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2017

Author dc:creator

• Jeong, Hyeon Soo

Contributors dc:contributor

• Rodriguez-Zas, Sandra Luisa

• Migraine
• Transcriptome
• Hyperalgesia
• Neuropeptides
• Transcription factor

Statement dc:rights

• Copyright 2017 Hyeon Soo Jeong

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/98230

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/98230