Estrogen Receptors: Interaction With Estrogen Response Elements and Estrogen Receptor-Associated Proteins

Since DNA-induced conformational changes in estrogen receptors affected recruitment of transcriptional coregulatory proteins, we examined differential recruitment of novel proteins. One novel protein was isolated and identified by protein sequencing as TAF-I beta, a protein previously implicated in acetylation, transcription and phosphorylation. TAF-I beta not only associates with ER in a cell environment, but also inhibits acetylation of histones and ER alpha in vitro and decreases ER alpha acetylation in vivo. Therefore, TAF-I beta may alter ER-mediated transcription by changing acetylation of ER or histones or both. Taken together, these data indicate that estrogen receptors associate with a variety of regulatory proteins and that interaction with these factors is affected both by an initial interaction with DNA and further modulated by receptor conformations induced by individual ERE sequences.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Molecular and Integrative Physiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Loven, Margaret Anne

Contributors dc:contributor

• Nardulli, Ann M.

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI3044163

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/87216