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Showing 1 to 13 of 13 for “"Protein sequencing"”.
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Protein sequencing using a combination of mass spectrometric methods
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1996.
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Enabling tools for de-novo single molecule protein sequencing
Proteins are the functional units of all living cells and the subsequent product of many genes. Subtle changes in protein structure and function can cause deleterious effects and, in cases of critical protein units, precipitate large-scale biological dysfunction. Amino acids are the fundamental …
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Single-Molecule Protein Sequencing (I) and Genetically Dominant mRNA Therapies to Combat Viral Evolution (II)
Proteins serve critical structural and dynamic functional roles at the cellular level of all living organisms. Understanding protein contribution to biological function is critical and rests on having appropriate technologies for quantification and identification. The central dogma of molecular …
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Strategies for Computational Protein Design with Application to the Development of a Biomolecular Tool-kit for Single Molecule Protein Sequencing
<p>One of the key properties of proteins is that they exhibit remarkable affinities and specificities for small-molecule and peptide binding partners. To improve the success rate of rational, computational protein design and widen the scope of potential applications, it is useful to define …
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Applications of mass spectrometry to protein characterization
The application of mass spectrometry as a protein sequencing method is demonstrated by the study of bovine kidney aldose reductase. The protein is blocked at the N-terminus and is not amenable to Edman degradation. Using a strategy of homology comparison, the amino acid sequence was determined …
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Proteomic Identification of Hemp (Cannabis sativa) Seeds by Tandem Mass Spectrometry and Evaluation of Cytotoxic Activity
… high-resolution ESI QTOF LC/MS/MS, and De Novo protein sequencing bioinformatics tools. The process involved defatting cannabis sativa seeds in hexane and extracting protein in PBS buffer over two days at 4°C. The extracted proteins were precipitated in 80% ammonium sulfate, dialyzed, and …
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Estrogen Receptors: Interaction With Estrogen Response Elements and Estrogen Receptor-Associated Proteins
… recruitment of transcriptional coregulatory proteins, we examined differential recruitment of novel proteins. One novel protein was isolated and identified by protein sequencing as TAF-I beta, a protein previously implicated in acetylation, transcription and phosphorylation. TAF-I beta not …
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From the Origins of Life to Epidemics: Bayesian Inference, Stochastic Simulation, and Dynamics of Bioinformatic Systems
With the advent of DNA and protein sequencing, X-ray crystallography, and other methods of analysis, researchers interested in biological questions have been presented with a wealth of data to parse and interpret. Additionally, some hypotheses, such as those pertaining to the origin of life, are …
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KSHV encoded viral processivity factor, ORF59, regulates multiple pathways for viral replication
… infected cells and identified ORF59 interacting proteins through protein sequencing and LC/MS spectrometry analysis. From a large number of ORF59 interacting proteins, we pursued few candidates for further study including host proteins Nucleolin and MCM4 in addition to viral protein, ORF57. Our …
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Urinary prothrombin fragment 1 : a potential role-player in the protection of South African blacks from calcium oxalate kidney stone disease
… presents a study of the inhibitory role of the protein, urinary prothrombin fragment 1 (UPTFI ), and its biochemical characterisation in both population groups. In a preliminary study, the urine composition and inhibitory activity of urine and urinary macromolecules from healthy white and black …
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Protein characterization using a computational microscope: Nanopore sequencing and biological condensates
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Structure, membrane association, and processing of meprin subunits
Meprins are oligomeric cell-surface metalloproteinases that are expressed at high concentrations in the renal brush border membranes of mice. Meprins consist of two types of subunits, α and β, which are the products of two different genes. The β subunit cDNA was cloned and sequenced from mouse …
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Characterization of protein microstructure by various chromatographic techniques
… new and reliable techniques to characterize proteins and biopharmaceuticals. In addition, characterization aids in understanding the intricate relationship between a protein's structure and its function. To address this challenge, two protein structural parameters, 1) amino acid surface area …