Integration and Excision Mechanism of Bacteroides Conjugative Transposons

The third project is about the regulatory mechanism of RteB on CTnDOT. RteA and RteB are shown to be global regulators that regulate many genes on Bacteroides host. Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are over-expressed in E. coli and partially purified. RteB is a soluble protein and gel shift result shows that RteB binds to 168bp upstream of rteC promoter region. However, the mutant did not bind to any probes tested. Footprint assay is required to determine the exact binding site on rteC promoter region.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Song, Bo

Contributors dc:contributor

• Abigail Salyers

• Biology, Microbiology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3314901

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86704